Speaker: Sonja Hess, Ph.D., MedImmune

Topic: In-depth proteomics characterization of differentiating cells using bottom-up and middle-down techniques

Date: Monday, November 20, 2017

Time: 6:15 pm Dinner, 7:15 pm: Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Stefani Thomas (sthoma92@jhmi.edu) if you will be attending the dinner.

Abstract: We were interested in studying differentiating cells using proteomics techniques. We used murine erythroleukemia (MEL) cells for our initial studies and then moved on to hematopoetic stem cells. Our investigations required a number of optimizations and innovations in our bottom-up and middle-down analytic pipelines. I will present our characterization of global and histone changes during the differentiation steps and show what insights can be obtained with the current state-of-the-art.