September 2022 Meeting and Vendor Night

Speaker: Ling Hao, George Washington University

Topic: Capturing Organelle Dynamics with Multifaceted MS-Omics Strategies

Date: Monday, September 19, 2022

Time: 6:00 pm Dinner and Vendor Night, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
This will be an in-person meeting. Attendees are required to show a vaccine card (either at the door or in advance using the web form).

Dinner: Please RSVP to Andy Qi (andy.yue.qi@gmail.com) by Friday, September 16th if you will be attending the dinner.

Abstract: Lysosomes and mitochondria are membrane-bound organelles in the cell that are responsible for trash-disposal and ATP production, respectively. Lysosomal and mitochondrial dysfunctions have been linked to numerous human diseases, such as neurodegeneration, cancer, and cardiovascular diseases. These organelles are highly dynamic and frequently interact with other cellular components in a transient fashion, which are difficult to capture with traditional immunoprecipitation and organelle isolation methods. Here, I will describe our recent efforts in developing proximity labeling proteomics, metabolomics, and dynamic SILAC approaches to characterize sub-organelle microenvironment and protein turnover in human iPSC-derived neurons. I will also
introduce our newly established universal and sample type-specific contaminant libraries that can benefit both DDA and DIA proteomics for the broad bottom-up proteomics community.

June 2022 Meeting

Topic: Post-ASMS Poster Night and ASMS Travel Award Presentations

Date: Monday, June 27, 2022

Time: 6:15 pm Dinner (outdoors) and ASMS posters, 7:30 pm Presentations

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
This will be an in-person meeting. Attendees are required to show a vaccine card (either at the door or in advance using the web form).

Dinner: Please RSVP to Dapeng Chen (cdpumd@gmail.com) by Friday, June 24th if you will be attending the dinner and/or if you will participate in the poster session.

ASMS Travel Award Recipients:

    • Haorong Li, George Washington University

: “Integrated Proteomics and Metabolomics of Mitochondrial Neurodegenerative Disease MELAS using Patient-derived Fibroblasts”

    • Hannah Lawther, Smithsonian Museum Conservation Institute

: “Minimally-Invasive Liquid Microjunction Sampling to Identify Dye on a 17th Century Spanish Illuminated Manuscript”

    • Jie Li, University of Maryland

: “Proteo-Metabolomics of Spemann’s Organizer in the Vertebrate (Frog) Embryo” and “Cell-lineage Based Multi-omics in the Vertebrate (Frog) Embryo”

May 2022 Meeting

Speaker: John Janiszewski, NIH/NCATS

Topic: An Open Port Sampling interface for High-throughput Mass Spectrometry:
Emerging Biotechnology for Screening and Reaction Optimization in Drug Discovery

Date: Monday, May 16, 2022

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
This will be an in-person meeting. Attendees are required to show a vaccine card (either at the door or in advance using the web form) . If you have submitted your vaccine card before, your status is already recorded.

Dinner: Please RSVP to Dapeng Chen (cdpumd@gmail.com) by Friday, May 13th if you will be attending the dinner.

Abstract: The Open-Port Sampling Interface (OPI or OPSI) presents a flowing solvent stream that can be adapted for direct sampling by mass spectrometry in a variety of formats. In Acoustic-Droplet ejection Mass spectrometry (ADE-MS) the OPI is positioned directly above an acoustic transducer such that nL sample volumes can be ‘ejected’ from 96- or 384-well plates directly into the OPI flow stream for direct ESI-MS/MS quantitative sampling. AE-MS has been applied in a variety of reaction screening applications in drug discovery. We report on our testing of the OPI in two sampling configurations. An AE-MS application that uses a modified EDC ATS-100 acoustic dispenser for High-Throughput screening of a biochemical (TMPRSS2) enzyme assay, and in vertical orientation for direct droplet sampling using a pneumatic aspiration-dispense pumping system. The pneumatic system delivers a pressure-pulse that can eject a 3µL drop cleanly and consistently (6% CV) for direct OPSI-MS sampling.

One week left! 2022 Young Investigator Travel Awards

2022 Washington-Baltimore MSDG Young Investigator Travel Awards

The Washington-Baltimore Mass Spectrometry Discussion Group (WBMSDG) is pleased to announce that applications are now being accepted for the 2022 Young Investigator Travel Awards. Awards will be granted to outstanding young investigators at the undergraduate or graduate student level to support travel to the 70th ASMS Conference in Minneapolis, MN. Undergraduate and graduate students in laboratories and institutions traditionally associated with the WBMSDG in the following geographic regions are encouraged to apply: from Richmond and Charlottesville, VA to the South and Newark, DE to the North. Three awards will be given. 1st place: $600, 2nd place: $500, 3rd place: $400.

Complete applications consist of the following items:
1. Travel Grant 2022 Application form (includes a checklist)
2. Electronic copy of ASMS abstract
3. Evidence of abstract acceptance by ASMS indicating the presentation format (poster or oral)
4. Curriculum Vitae or Resume
5. Two-page summary of research project (figures can be included)
6. Letter of recommendation from advisor

Applicants should submit Items 1-5 listed above as a single PDF file to Dr. Casey Daniels. Item 6 must be sent directly by the applicant’s advisor to Dr. Casey Daniels:

Dr. Casey Daniels
WBMSDG co-chair
Email: casey.daniels@astrazeneca.com

The deadline for applications is 5 PM EST on Friday, May 6th, 2022. A panel of WBMSDG members will act as reviewers. Please note, previous winners are encouraged to apply if the award application for the upcoming ASMS conference significantly differs from the previously successful application. In the event that ASMS is cancelled, awards will be given out as well as prize amounts up to the full award to cover any incurred costs associated with ASMS travel. Successful applicants will be expected to give a 10-minute oral presentation at the post-ASMS WBMSDG meeting on June 20th, 2022 at Shimadzu Scientific in Columbia, MD.