Speaker: Kyle Anderson, National Institute of Standards and Technology

Topic: Advancements in methods and instrumentation for HDX-MS and LC-MS

Date: January 13, 2025

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Dingyin Tao (owendtao@gmail.com) by Friday, January 10 if you will be attending the dinner.

Abstract: Subzero temperature chromatography minimizes loss of deuterium during HDX-MS analysis. We developed an HDX-MS chromatography apparatus capable of performing long analytical separations at -30 °C. The apparatus has precise temperature control with two enzyme column compartments held at independent temperatures and allows for rigorous cleaning in parallel to data acquisition to reduce instrument downtime and sample carryover. Methods for both reversed phase and HILIC analytical separations at subzero temperature were developed. HILIC methods developed greatly reduced operational backpressures for easier adoption using any HPLC pumps and enabled virtually water-free separations at subzero temperature. An HPLC pump delivering custom wash solutions to immobilized protease columns was added to replace conventional injections of common wash solutions, which greatly reduce sample carryover and cut necessary blank runs from 5 to 1 for an IgG1 digest. Additionally, methods for automated online removal of phospholipids from membrane proteins will be presented.

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Lightning Talk
Jacob Epstein (NIH/NIA/IRP)

Speaker: Berk Oktem, Food and Drug Administration

Topic: Role of Mass Spectrometry in Chemical Analysis of Medical Device Extractables

Date: December 16, 2024

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Dingyin Tao (owendtao@gmail.com) by Friday, December 13 if you will be attending the dinner.

Abstract: Chemical analysis of medical device extracts has two main purposes: identification of unknown medical device constituents and quantification of known or unknown extractables. Mass spectrometry is an essential part of chemical analysis of medical device extractables. Identification of the extractables is an ongoing challenge with limited options. Quantification is also an important task in this field as the toxicologists, who need this chemical analysis data, make important decisions based on the both the identity and the reported quantities of the extractables. This presentation will attempt to offer a summary of the current status, ongoing research and challenges related to mass spectrometry in this field, and also lists future directions

Speaker: Ed Sisco, National Institute of Standards and Technology

Topic: What’s in My Drugs? – Using Ambient Ionization Mass Spectrometry to Gain Near Real-Time Insights into the Illicit Drug Supply

Date: November 18, 2024

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Dingyin Tao (owendtao@gmail.com) by Friday, November 15 if you will be attending the dinner.

Abstract: Drug overdoses remain near all-time highs, driven by the continued prevalence of synthetic opioids coupled with a constantly changing drug supply. Keeping pace with the drug supply makeup using traditional approaches (i.e., forensic laboratories or toxicology testing) is difficult due to backlogs and lagging spectral libraries. To address these challenges, NIST has worked to establish the Rapid Drug Analysis and Research (RaDAR) program which provides public health and law enforcement entities across the country access to rapid (24 hour) turnaround, comprehensive drug testing using drug paraphernalia residue and ambient ionization mass spectrometry (AI-MS). In this presentation I will discuss our efforts to develop AI-MS methods, libraries, and algorithms that have enabled this measurement capability, what data we are generating and how it is being used, and outstanding data analytic challenges we face. In addition, I will touch on our ongoing research efforts to develop rapid quantitation methods for drug samples as well as a new initiative to bring high-resolution AI-MS to the field through a mobile laboratory platform.
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Gold Sponsor Lightning Talk
Karen Luo, Mid-Atlantic MS Product Specialist
Agilent Technologies, Inc.

Speaker: Neil L. Kelleher, Northwestern University

Topic: Digitizing Proteoform Biology with Single Molecule & Single Cell Mass Spectrometry

Date: Monday, September 16, 2024

Time: 6:00 pm Dinner and Vendor Night, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions) This will be an in-person meeting.

Dinner: Please RSVP to Dingyin Tao (owendtao@gmail.com) by Friday, September 13 if you will be attending the dinner.

Abstract: Since the completion of the Human Genome Project, much has been made of the need to bridge the gap from genes and traits. As a key nexus for the many interacting ‘-omes’ (genome, transcriptome, proteome, metabolome, etc.), the proteome should offer a tight link between genotype and phenotype. Proteoforms, or all of the precise molecular forms of a protein, capture all sources of variability in protein composition (i.e., SNPs, isoforms, post-translational modifications), and thus provide crucial insights into regulation and function. Now, “single ion” mass spectrometry is poised to convert genes to proteoform signatures at a far faster rate. Recently we developed proteoform imaging mass spectrometry (PiMS), with individual ion mass spectrometry. This platform has been extended now to single-cell Proteoform imaging Mass Spectrometry (scPiMS), boosting cell processing rates by >20-fold in the field while detecting proteoforms from single cells.

Topic: Post-ASMS Poster Night and ASMS Travel Award Presentations
All attendees are invited to put up an ASMS poster

Date: Monday, June 17, 2024

Time: 6:15 pm Dinner and ASMS posters, 7:30 pm Presentations

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Jonathan Ferguson (jonathan.ferguson33@gmail.com) by Friday, June 14 if you will be attending the dinner.

ASMS Travel Award Recipients:

• • Melissa Leyden, University of Virginia
    

: “Characterization of Insect Sperm Nuclear Basic Proteins by Liquid Chromatography – Tandem Mass Spectrometry”

• • Juliet Obi, University of Maryland Baltimore
    

: “A Structural and Dynamic Basis for the Interactions of the Dengue Nonstructural (NS5) Protein with Stem Loop A (SLA)”

• • Bay Xu, Johns Hopkins University

: “SPOT: Spatial Proteomics through On-site Tissue-protein-labeling”