Sunday, December 16th, 2018

Meetings

December 2018 Meeting

Speaker: Amina Woods, The National Institute on Drug Abuse Intramural Research Program

Topic: Tracking the Progression of Traumatic Brain Injury and the Therapeutic Response to a Peptide Drug Using Innovative Imaging MS

Date: Monday, December 17, 2018

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instruments, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Yan Wang (yanwang@umd.edu) by Friday, December 14th if you will be attending the dinner.

Abstract: Traumatic brain injury (TBI) is a serious public health problem and a leading cause of death in children and young adults. It also contributes to a substantial number of cases of permanent disability. As lipids make up over 50% of the brain mass and play a key role in both membrane structure and cell signaling, their profile is of interest. In this study, we show that advanced mass spectrometry imaging (MSI) has sufficient technical accuracy and reproducibility to study the progression of TBI as well as a therapeutic response. The anatomical distribution of 50 μm diameter microdomains demonstrate changes in brain lipid levels in a rat model of controlled cortical impact (CCI) 3 days post injury with and without treatment.

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Bruker Seminar

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November 2018 Meeting

Speaker: Daniele Fabris, University at Albany

Topic: MS-based approaches for the elucidation of nucleic acid higher-order structure and dynamics

Date: Monday, November 19, 2018

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instruments, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Yan Wang (yanwang@umd.edu) by Friday, November 16th if you will be attending the dinner.

Abstract: The discovery of ribozymes and riboswitches has keenly reasserted the critical role played by higher-order structure in determining the function of nucleic acid sequences that do not code for actual proteins. Mass spectrometry-based approaches can provide valuable information about base-pairing and longrange interactions, which define the secondary, tertiary, and quaternary structure of nucleic acids. We have been developing strategies based on high-resolution and ion mobility spectrometry (IMS) mass spectrometry to investigate the structure-function relationships in noncoding viral RNA. We have demonstrated that the concerted application of footprinting and crosslinking methods can provide valid spatial constraints for modeling operations, leading to the solution of actual 3D structures. Top-down strategies can provide direct information on the position and strength of base-pairing interactions that stabilize higher-order structure. Putative structures are corroborated by IMS determinations that reveal the global topology of target RNA. These approaches constitute a valuable alternative for the investigation of systems that, owing to their large size and flexibility, are not directly amenable to classic high-resolution techniques employed in structural biology. Their implementation has been providing new insights into the processes of genome recognition, dimerization, and packaging of HIV-1 and other retroviruses, which have the potential of leading to the development of novel therapeutic strategies.

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