Date: July 2-8, 2017
The 11th Edition of the Summer School for “Mass Spectrometry in Biotechnology and Medicine” (MSBM), will be held at the Centre for Advanced Academic Studies (CAAS), Dubrovnik, Croatia, 2-8 July 2017. Once again MSBM features instruction by the leading international scientists in mass spectrometry. However, this year MSBM is working together with the Nederlandse Vereniging voor Massaspectrometrie (NVMS) to host the third International Mass Spectrometry School (IMSS-2017) on behalf of the International Mass Spectrometry Foundation (IMSF).
Speaker: Robert N. Cole, Johns Hopkins School of Medicine
Topic: Adventures of Capillary Electrophoresis in Proteomics, from Top-down to Bottom-up
Date: Monday, March 27, 2017
Time: 6:15 pm Dinner, 7:15 pm: Presentation
Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
Dinner: Please RSVP to Katherine Fiedler (Katherine.L.Fiedler@fda.hhs.gov) before February 27 if you will be attending the dinner or are a presenting as a vendor.
Abstract: Capillary zone electrophoresis (CE)-MS has become more accessible with robust electrospray interfaces, presenting a potential alternative to LC-MS. CE achieves rapid and high resolution analyte separation based on net charge and hydrodynamic volume. CE is orthogonal to reversed phase LC with additional benefits of low carryover, low (nanogram) sample injection, and rapid sample loading. CE-MS analysis of complex peptide mixtures is challenging due to the seconds-wide elution peaks produced during the rapid separations. However, increases in MS data acquisition speeds warrant re-examination of the role of CE in proteomics. We are evaluating a microfluidic chip-based CE interface (ZipChip, 908 Devices Inc) for top-down analysis of intact proteins, analysis of high charge/high pI peptides, and bottom-up quantification of proteins. Even with major challenges of sample concentration, protein complexity and automation, CEMS has a bright future in protein analysis.Read more of this article
Date: March 23rd, 2017Read more of this article