Wednesday, May 4th, 2016

Meetings

Notices

1.May 9, 2016 WBMSDG Meeting in Columbia; Speaker:Peter Nemes, Ph.D., George Washington University; Topic:Uncovering Molecular Cell Heterogeneity in the Cleavage-Stage Vertebrate Embryo using Single-cell Mass Spectrometry

2.2016 Washington-Baltimore MSDG Young Investigator Travel Awards; Application deadline April 25, 2016

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May Meeting

Speaker: Peter Nemes, Ph.D., Assistant Professor, Department of Chemistry, The George Washington University

Topic: Uncovering Molecular Cell Heterogeneity in the Cleavage-Stage Vertebrate Embryo using Single-cell Mass Spectrometry

Date: Monday, May 9, 2016

Time: 6:15 pm: Dinner and Social Hour; 7:15 pm: Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner and Social Hour Please RSVP to Jace Jones(jjones@rx.umaryland.edu) if you will be attending dinner.

Abstract: How differential gene expression establishes tissue-specific cell differentiation during normal embryonic development is not fully understood, because it has been technologically challenging to measure broad types of proteins and metabolites in single embryonic cells. Although mass spectrometry is the method of choice for the discovery measurement of these molecules, typical workflows in the field combine a large cohort, often millions, of cells to improve detection performance. Because pooling inherently averages out the analytical signal among components of the sample, chemical information specific to each cell is lost. In this talk, we will explore a single-cell mass spectrometry technology we have recently developed to compare the molecular state of single embryonic cells in the early developing (cleavage-stage) vertebrate embryo. We use the South African clawed frog (Xenopus laevis), a powerful model in cell and developmental biology, to validate the single-cell mass spectrometry platform. Next, we use the platform to compare the metabolic and proteomic activity of embryonic cells that give rise to different types of tissues from the 16-cell Xenopus embryo. Finally, we present targeted experiments, in which new developmental roles are discovered for small molecules. Single-cell mass spectrometry opens new investigative avenues for cell and developmental biology to help better understand the molecular players of normal development.

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April Meeting

Speaker: Stephen E. Stein, Ph.D., Biomolecular Measurement Division, NIST

Topic: Mass Spectral Libraries of Everything

Date: Monday, April 11, 2016

Time: 6:15 pm: Dinner and Social Hour; 7:15 pm: Presentation; (Student Presentation: 7:10 pm; TBA)

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner and Social Hour Please RSVP to Jace Jones(jjones@rx.umaryland.edu) if you will be attending dinner.

Abstract: Libraries of mass spectra of known compounds have long been used for identifying compounds in GC/MS experiments and are finding increased use in tandem LC/MS experiments, especially in metabolomics and proteomics studies. Even given the substantial coverage of current libraries, a large fraction of the spectra remain unidentified. In some areas only a small fraction of components can be identified and even for well-developed areas (gc/ms of urine, for example), the origin of many spectra is unknown and likely to remain so for some time. To characterize these unknowns, we have been involved in building annotated libraries of good quality, but unidentified spectra in gc/ms and lc/ms experiments. Progress in three areas will be discussed: 1) GC/MS – multiple occurrences of spectra at fixed retention indices have been collected, clustered and annotated for a range of materials including urine, essential oils and various NIST reference materials; 2) LC/MS proteomics – we have constructed extensive libraries of unidentified peptide from proteomics analysis and developed special methods for their annotation and identification; 3) LC/MS metabolomics – we have built an collection of unidentified spectra from NIST urine reference materials and developed tools for organizing and identifying them. Illustration of the use of these libraries alongside more conventional libraries of reference spectra of identified compounds will be presented.

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