Wednesday, July 30th, 2014

Meetings

Notices

1.June 23, 2014 Meeting in Columbia; Post-ASMS Poster Session and Dinner

2. 2014 Washington-Baltimore MSDG Young Investigator Travel Awards

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June Meeting

Topic: Post-ASMS Dinner and Poster Session/strong>

Date: Monday, June 23, 2014

Time: 6:15 pm: Dinner and Posters; 7:15 pm: Travel Award Recipient Presentations

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Please RSVP to Christopher Crutchfield (Crutchfield@jhmi.edu) if you will be attending dinner or presenting a poster.

2014 MSDG Travel Award Recipients:

Meghan Burke, UMD, PI: Catherine Fenselau
Stacy Malaker, UVA, PI: Donald Hunt
Alison Scott, University of Maryland, Baltimore, PI: Robert Ernst
Sylwia Stopka, The George Washington University, PI: Akos Vertes

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May Meeting

Speaker: Dr. Amanda Hummon, University of Notre Dame

Topic: Characterizing the Proteome of 3-Dimensional Cell Cultures

Date: Monday, May 12, 2014

Time: 6:15 pm: Dinner and Social Hour; 7:15 pm: Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner and Social Hour Please RSVP to Christopher Crutchfield (Crutchfield@jhmi.edu) if you will be attending dinner.

Abstract:

3D cell cultures are valuable in vitro model systems for biological research. While simple and quick to grow, 3D cultures recapitulate the physiology of their source tissue. For example, when cancerous cell lines are grown in 3D, the resulting structures capture many of the molecular and pathophysiological aspects of tumors. These cultures provide higher-throughput, more cost-effective, and quicker alternative model systems than animals. We have developed an approach to examine endogenous protein distributions in 3D cell cultures via MALDI imaging mass spectrometry (MALDI-IMS). We are currently expanding our methodology to examine the uptake and penetration of clinically administered drugs into the 3D structures. While we developing our approach with a well-known drugs, our approach can be expanded to novel therapeutics. We have examined analyte distributions in 3D cultures of the human colon carcinoma cell line, HCT 116. We observed m/z values differentially expressed across the HCT 116 cultures by MALDI imaging mass spectrometry. For example, we detected multiple species distributed across the entire structures, while a species corresponding to 12828 Da was detected predominately in the necrotic center cells. Using MS/MS approaches, we have identified specific proteins localized to distinct regions of the 3D structures. We are currently generating inducible cell lines to knockdown expression of key cell adhesion proteins implicated in metastasis. With these cell lines, we will reduce expression of cell adhesion proteins in our 3D cultures. We will then map the resulting changes in protein distribution and abundance with IMS as we model the metastatic process in vitro. In tandem, we have also characterized the proteomic and phenotypic response of the 3D cultures to drug treatments. Beginning with the drugs Oxaliplatin and Irinotecan, we treated the 3D cultures and evaluated their phenotypic changes to determine an ideal dose for our studies. We then mapped the distribution of these two drugs in treated cultures. We also performed LC-MS/MS studies on separate cultures to determine metabolites produced with treatment. We have demonstrated that while Irinotecan fully penetrates colon tumors models, Oxaliplatin does not fully treat the cancerous cells. Using IMS, we mapped the distributions of both the drug and metabolites via IMS. We are currently assessing the proteomic and metabolic changes that accompany drug treatment in these cultures.

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