Topic: Chromatography and Mass Spectrometry of Intact Proteins
Speaker: Dr. Steven M. Musser, Director, Office of Regulatory Science, Center for Food Safety and Applied Nutrition/FDA, College Park, MD
Time: 7:30 pm; Social Hour: 6:30 pm
Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046
Click here for Meeting Directions
Dinner: Please join co-chairs Alexei Gapeev, Berk Oktem and the speaker for dinner at for dinner at the Ram’s Head Tavern at Savage Mill, 8600 Foundry Street, Savage, MD 20763 at 5:00 pm. Contact Alexei Gapeev (gapeev@umbc.edu ) and let him know you will be there.
Abstract: Although LC/MS of peptides has advanced a great deal, with numerous effective sample preparation techniques, chromatography columns, and automated MS routines, the same can’t be said for the analysis of intact proteins. While peptide analysis is a powerful tool, missed cleavages and incomplete sequence coverage make it difficult to use for the comparison and differentiation of closely related proteins, such as protein toxins, food allergens, and similar bacterial strains. For this reason, we are exploring techniques to effectively extract, separate, and analyze intact proteins, with specific attention to parameters that permit high protein recoveries, reproducible results, and maximize the total number of cellular proteins observed.
COURSE DESCRIPTION: The Washington-Baltimore Mass Spectrometry Discussion Group (WBMSDG) will present a short course on “Interpretation of Mass Spectra.” This introductory level course on the qualitative interpretation of mass spectra of organic, biochemical and environmental compounds will be taught by solving practical examples. Registrants should be familiar with basic principles of organic chemistry. The principles of interpretation will be introduced through lectures and augmented through extensive problem-solving. Although emphasis will be placed on the interpretation of electron ionization mass spectra, the concepts of chemical ionization, electrospray ionization, and MALDI will also be introduced. The workshop format and a high instructor:student ratio are designed to benefit those with little or no experience, but who wish to expand their interpretation skills. This is the same short course that the WBMSDG presents each year at the ASMS Annual Conference.
FORMAT A workshop format and limited class size will ensure the participants ample opportunity to interact with the instructors.
WHO SHOULD ATTEND: Chemists and Laboratory Personnel with no formal training in mass spectrometry, and Chromatographers who are using mass selective detectors or ion traps as chromatography detectors. A knowledge of basic organic chemistry will be assumed.
DATE/PLACE: April 6 and 7, 2010 - 8:30 a.m. to 5:00 p.m. (ample free parking available)
Shimadzu Scientific Instruments, Inc., 7100 Riverwood Drive, Columbia, Maryland 21046. Directions: for driving directions and map, see:Â http://wbmsdg.org/wordpress/
REGISTRATION: Early registration is recommended and encouraged! Course enrollment will be limited to 36 students to ensure a high instructor to student ratio. Registration fee includes textbooks, course materials, lunches and coffee breaks. To register, complete and return the attached Registration Form.
Registration Fee
If payment received by March 15, 2010: $ 400 (full); $ 250 (student)
If payment received after March 15, 2010:$ 425 (full);Â $ 275 (student)
*Students must be enrolled full-time in a degree program up to and including the Ph.D. and must attach verification of current full-time student status by providing both a copy of your student I.D. and a letter from your department chairman or research advisor on university stationery verifying your student status.
A non-refundable charge of $95 will apply to all cancellations received in writing before March 15, 2010, and will be processed after the course. No refunds will be made after that date. Registrants who are unable to attend may send a substitute and must immediately contact the Course Manager.
CEU’s Course rated as 1.5 Continuing Education Units (CEUs)
CONTACT Ms. Janet Cunningham, Course Manager; Barr Enterprises, P.O. Box 8032, West Grove, PA 19390
Phone 301-668-6001; e-mail janetbarr@aol.com
