Meetings

Notices

January 23, 2012 MSDG Meeting at Shimadzu Training Center in Columbia: Speaker; Dr. Stuart Maudsley, NIA/NIH; Topic: Rapid and Enhanced Proteolytic Digestion using Electric-Field-oriented Enzyme Reactor

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January MSDG Meeting

Speaker: Dr. Stuart Maudsley

Topic: Rapid and Enhanced Proteolytic Digestion using Electric-Field-oriented Enzyme Reactor

Date: Monday, January 23, 2012

Time: 7:30 pm

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please join the speaker and the co-chairs for dinner at the Ram’s Head Tavern at Savage Mill, 8600 Foundry Street, Savage, MD 20763 at 5:30 pm.  Contact Shelley Jackson (Shelley.Jackson@nih.hhs.gov) to let him know you will be there for dinner.

Abstract: We have created a novel enzyme reactor using electric field-mediated orientation and immobilization of proteolytic enzymes (trypsin/chymotrypsin) on biocompatible PVDF membranes in a continuous flow-through chamber. Using less than 5 minutes, this reactor in various enzyme combinations can produce enhanced rapid digestion for standardized prototypic proteins, hydrophilic proteins and hydrophobic transmembrane proteins when compared to in-solution techniques. With improved digestive efficiency, our reactor improved the overall functional analysis of lipid raft proteomes by identifying more closely functionally linked proteins and elucidated a richer set of biological processes and pathways linked to the proteins than traditional in-solution methods.

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December MSDG Meeting

Speaker: Amina Woods, Ph.D.

Topic: Molecular Imaging of Brain Lipids in Health and Disease

Date: Monday, December 19, 2011

Time: 7:30 pm

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please join the speaker and the co-chairs for dinner at the Ram’s Head Tavern at Savage Mill, 8600 Foundry Street, Savage, MD 20763 at 5:30 pm.  Contact Shelley Jackson (Shelley.Jackson@nih.hhs.gov) to let him know you will be there for dinner.

Abstract:

Lipids are a major component of brain tissue, accounting for almost half of the brain dry weight. They are major building blocks of biological membranes and also play an important role in cell signaling and cell death. Altered levels of lipids in brain tissue are associated with diseases such as Alzheimer’s, Tay-Sachs, and Niemann-Pick disease. MALDI-MS methods have been developed for the in situ analysis of biomolecules, typically peptides and proteins, from tissue. We used MALDI-MS for direct analysis and imaging of lipids in tissue. We have advanced the field of imaging as our work not only maps the distribution of lipids in various areas of the brain it also gives detailed structural information with histological accuracy.  After mapping the content and distribution of lipids in the normal brain, we have demonstrated the changes that occur over time in the lipid make up of the brain in blast induced and mechanical brain injury in animal models.

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