Special guest speaker seminar on Tuesday, 7/31/18 at NIH in Bethesda, MD: room 4-433, 2-3 pm.
Prof. Dr. Lihua Zhang
National Chromatographic R. & A. Center
Dalian Institute of Chemical Physics, Chinese Academy of Sciences
“New Methods For Proteome Analysis, From Quantitation To Interaction”
Due to the complexity of samples, with the wide dynamic range in abundance, the diverse physical chemical properties, and the changes with time and space, the development of novel methods for proteome analysis is imperative.
In our recent work, ionic liquid, 1-dodecyl-3-methylimidazolium chloride, was selected to improve the solubility of proteins, especially for hydrophobic proteins, and simultaneously ensure the compatibility with the subsequent tryptic digestion and MS detection. Furthermore, ionic liquid based filter assisted sample preparation (i-FASP) protocol was proposed, and demonstrated the superiority to the commonly used SDS or urea based FASP method, to achieve the deep coverage proteome qualitation and quantitation.
A fully automated sample treatment (FAST) device was designed, by which proteome samples, no matter for proteins extracted from cells, tissues or plasma, could be on-line denatured, alkylated and digested within 20 min. Moreover, FAST could be integrated with any commercial LC-MS/MS instrument, to achieve the automated proteome quantitation, by which the throughput, accuracy, precision and coverage was improved obviously, compared with the off-line multi-step operation, especially for label-free quantitation.
Furthermore, various novel chemical crosslinkers were designed to achieve the comprehensive study on protein-protein interaction.
All the above mentioned methods have been applied to achieve the deep coverage proteome analysis in various fields, such as life science, precision medicine and food safety.