Category Archives: Training

UMBC Proteomics/mass spectrometry Workshop

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The UMBC proteomics/mass spectrometry facility is hosting a hands-on workshop on mass spectrometry tools for proteomics. The workshop is taking place February, 25-27 and will center on the facility nanoLC-MS system. Participants will learn underling theory of mass spectrometry, liquid chromatography and protein database searching approaches. The hands-on training on the instrument operation will supplement theory.

To register, contact: Dr. Alexander Gapeev, Manager, MS Facility, Department of Chemistry and Biochemistry, University of Maryland, Baltimore County

gapeev@umbc.edu.   See the MSDG web site for further details.

December 15, 2008 WBMSDG Meeting

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Speaker: Dr. Justin Hettick; CDC NIOSH in Morgantown

Topic: Discrimination of microorganisms by MALDI-TOF MS

Abstract: Unambiguous identification of microorganisms such as bacteria or fungi is essential for effective surveillance and treatment of disease.  Unfortunately, diagnosis by classical microbiological methods often requires lengthy culture procedures or subjective micro-and macroscopic evaluations.  To stem the spread of disease, rapid procedures for the accurate identification of microorganisms are necessary.  To that end, we have been developing mass spectrometry-based tools for the analysis of bacteria and fungi.

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOF-MS) can be used to generate highly reproducible, characteristic “fingerprint” mass spectra.  These “fingerprint” mass spectra may be reduced to a subset of significant mass-to-charge values through statistical tools and compared against a reference library.  Using this technique, microorganisms can be easily identified at the species and strain level with error rates approaching zero.

Time: 7:30 pm

Location: Shimadzu Scientific Instruments, Inc. Training Center, 7100 Riverwood Drive, Columbia, MD 21046

Directions: Shimadzu Scientific Instruments

Mass Spec in BioTech Workshop

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Date: October 20-22, 2008.
Location: UMBC
Contact: Alexei Gapeev, Ph.D.; University of Maryland, Baltimore County Dept. of Chemistry and Biochemistry 1000 Hilltop Circle Baltimore, MD 21250 office (410)455-6251 fax (410)455-2608 gapeev@umbc.edu

UMBC mass spectrometry facility is hosting a workshop on mass spectrometry tools for proteomics. The workshop will be held October 20-22 at the main UMBC campus. It will center on the facility nanoLC-MS system. The participants will learn underlying theory of mass spectrometry, liquid chromatography and protein database searching approaches. Hands-on training on the instrument (ThermoFisher LTQ) operation will supplement theory.

Expected outcome: The main goal of the workshop is to provide enough knowledge and hands-on experience so that, upon completion, attendees are able to independently plan experiments, prepare adequate samples, use the instrument in a productive way, recognize problems, conduct database search and interpret experimental results.

Who should attend: Prospective users of the recently installed nano-LC-MS system. Those interested in learning the mass spectrometry based proteomics. The workshop agenda is below: Day 1. Morning (9:30-12 am) and afternoon (1:30-2:30 pm): Instrument operation demonstration. Lecture. Nano-LC front end operation. Introduction to mass spectrometry as applied to the instrument and the discipline of proteomics. Day 1. Afternoon (2:30-4:30 pm):Hands-on exercise: Setting LC protocol and simple MS run. Setting up the instrument and software options. Setting up and running sequences. Day 2. Morning (9:30-12 am): Lecture. Topics include: Ion formation in the ESI source. Ion transmission and detection basics. MS scan modes (full scan, MS/MS, data dependent) and their utilization in mass spectrometry experiments. Protein ID workflow. Day 2. Afternoon: Hands-on exercise. Setting up a data dependent run. Data analysis software: Using software to display data, make annotations, manipulate other display options (e.g. ranges, background subtraction), export to other applications. Day 3. Hands on exercise. Database searching using BioWorks (Sequest) to mine for proteomics information. This section will include the sequest process overview, topics on obtaining a database, creating a database subset, indexing a database to speed up a search, setting specific parameters, interpreting the search data. The participants will analyze data acquired in day 2 and correlate data quality with the methods used.

Bruker Daltronics 2007 Mass Spectrometry Seminars

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New Mass Spectrometry Based Methods for Intact Protein and
Peptide Characterization in Biomarker Research

You are invited to discover how mass spectrometry is advancing the state of the art in protein function and biomarker research. Electrospray Ionization (ESI) and Matrix Assisted Laser Desorption Ionization (MALDI) mass spectrometry are the basis of important new tools that facilitate the characterization of peptides and proteins with high specificity. Complementary techniques are gaining in popularity for biomarker research and discoveries using MALDI imaging and novel ESI-MS/MS fragmentation methods.
Join our seminars to find out more about these emerging new technologies.

For more detail view the attached PDF. bdal_seminars20071

Visit www.brukerseminars.com for more information and registration.

Seminar Series 1
February 6 (9-12 AM): Bethesda, MD
February 7 (9-12 AM): Wilmington, DE
February 8 (9-12 AM): Research Triangle Park, NC

Seminar Series 2
February 20 (12-4 PM): New York City, NY
February 21 (9-12 PM): West Orange, NJ
February 21 (3-6 PM): E. Brunswick, NJ
February 22 (9-12 PM): King of Prussia, PA

Interpretation of Mass Spectra

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Short Course designed for Chemists and Laboratory personnel with no formal training in mass spectrometry, and Chromatographers who are using mass selective detectors or ion traps as chromatography detectors

Date: May 6 – 7, 2003 Germantown, Maryland

Course Description: The Mass Spectrometry Discussion Group of the Greater Washington-Baltimore Area will present a short course on “Interpretation of Mass Spectra.” This introductory level course on the qualitative interpretation of mass spectra of small organic, biochemical and environmental compounds will be taught by solving practical examples.

Registrants should be familiar with basic principles of organic chemistry. Extensive problem-solving time will be augmented by lectures to illustrate the principles of interpretation. Emphasis will be placed on the interpretation of electron ionization (EI) mass spectra. The workshop format and a high instructor-to-student ratio are designed to benefit those with little or no experience, but who wish to expand their interpretation skills.

Format: A workshop format and limited class size will ensure the participants ample
opportunity to interact with the instructors.

Who Should Attend: Chemists and Laboratory Personnel with no formal training in mass spectrometry,
and Chromatographers who are using mass selective detectors or ion traps as chromatography detectors. A knowledge of basic organic chemistry will be assumed.

Date: May 6 and 7, 2003 — 8:30 a.m. to 5:00 p.m. (ample free parking available)

Place: Agilent Technologies, 20403 Century Blvd., Germantown, MD 20874. Directions:
Take I-270 to Exit 16 and head towards Germantown, turn left onto Crystal Rock Dr.,
left on to Clover Leaf Center Dr., and left onto Century Blvd., building is on left.

Registration: Course enrollment will be limited to 24 students to ensure a high instructor-to- student
ratio. Registration fee includes textbooks, course materials, lunches Register early space is limited!

To register, complete and return the Registration Form in the attached PDF.ms-shortcourse-flier
CEU’s Course rated as 1.3 Continuing Education Units

Contact: Ms. Janet Cunningham, Course Manager
Barr Enterprises, P.O. Box 279, Walkersville, Maryland 21793
Phone 301-668-6001; Fax 301-668-4312; e-mail janetbarr@aol.com