Speaker: Kristine Glunde, Johns Hopkins University
Topic: Toward MALDI Microscopy: FluoMALDI, RaMALDI, and Single Cells
Date: November 13, 2023
Time: 6:15 pm Dinner, 7:15 pm Presentation
Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions) This will be an in-person meeting.
Dinner: Please RSVP to Jonathan Ferguson (firstname.lastname@example.org) by Friday, November 10 if you will be attending the dinner.
Abstract: The spatial resolution of matrix-assisted laser desorption/ionization (MALDI) imaging has significantly improved over the years and has now reached single cell resolution on commercial imaging mass spectrometers. This opens new possibilities of combining MALDI imaging with optical microscopy technologies including Raman and fluorescence microscopy. This talk will discuss our recently developed RaMALDI and FluoMALDI applications, which are streamlined, integrated, multimodal imaging workflows of Raman and fluorescence microscopy with MALDI imaging, performed on a single tissue section with one sample preparation protocol. In these projects, we discovered that co-crystallization of fluorophores with MALDI matrices significantly enhances fluorophore brightness, enabling the amplification of innate tissue autofluorescence and exogenous fluorophores. FluoMALDI and RaMALDI will advance structural-functional microscopic imaging in cell biology, biomedicine, and pathology.
WBMSDG has decided to include a lightning talk before the main talk at each event. Each lightning talk lasts 7 minutes with no more than 5 slides, and 5 minutes for Q&A. The desired speakers are early-career researchers/scientists in mass spec-related areas. They can be junior scientists, postdoctoral fellows, graduate or undergraduate students.
Please submit abstracts to co-chairs to apply for the lightning talks, and our board members will review the abstracts on a rolling basis.
Structure the abstract (maximum 300 words) with the following headings:
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