4th Annual North American Mass Spectrometry Summer School

4th Annual North American Mass Spectrometry Summer School
June 20-23, 2022, Madison, WI

Join us for our fourth annual mass spectrometry summer school. We are proud to have assembled over a dozen world leading experts in mass spectrometry for this three-day course. Our goal is to provide our students, both from academia and industry, an engaging and inspiring program covering the latest in the application of mass spectrometry to omic analyses. Tutorial lectures range from mass analyzers to the basics of data analysis. This program is made possible by generous funding from the National Science Foundation (Integrated Organismal Systems, Plant Genome Research Program, Grant No. 2010789) and the National Institutes of Health National Center for Quantitative Biology of Complex Systems (P41 GM108538). There is no cost to participate.

Registration closes on April 1, 2022: https://www.ncqbcs.com/resources/training/summer-school.

Expert Instructors:
Scott McLuckey | Purdue University
Rachel Loo | University of California-Los Angeles
Jenny Brodbelt | University of Texas-Austin
Joshua Coon | University of Wisconsin-Madison
Shawnna Buttery | STAR Protocols, Cell Press
Jesper Olsen | University of Copenhagen
Lingjun Li | University of Wisconsin-Madison
Jürgen Cox | Max Planck Institute of Biochemistry
Edward Huttlin | Harvard University
Ben Garcia | Washington University
Evgenia Shishkova | University of Wisconsin-Madison
Jessica Prenni | Colorado State University
Vicki Wysocki | Ohio State University
John Bowden | University of Florida

Tandem MS
Mass analyzers
Data acquisition
Data analysis
Experimental design
Top-down/Native MS
Spectral Intrepretation

February 2022 Virtual Meeting

Speaker: David Goodlett, University of Victoria

Topic: Lipid A as a Therapeutic and Diagnostic

Date: Monday, February 21, 2022

Time: 6:45 Virtual Social, 7:15 pm Presentation

Location: See Zoom invite in email on February 10 and 17 (sign up)

Abstract: Lipid A is the membrane anchor for Gram-negative bacteria that holds the much larger lipopolysaccharide (LPS) molecule in place in the outer membrane. Importantly in mammals, Toll receptor 4 (TLR4) recognizes lipid A the result of which is activation of a cytokine cascade that can aid the host in clearing the infection or if unchecked lead to a deadly cytokine storm. There are a range of activities from agnostic to antagonistic that are directly related to structure (e.g. Li). To exploit this we are working to better define the lipid A structure activity relationship for use as a vaccine adjuvants and antisepsis therapeutics (e.g. Scott). We are also using lipid A and related Gram-positive molecules to identify bacteria direct from source in under an hour (Leung) and have recently developed an antibiotic susceptibility test that works in concert with microbial identification providing results a day faster than existing methods. At the ICCVS in Gdansk we are interested in investigating the classic use of bacterial extracts as an immunotherapy (i.e. Coley’s toxins late 1800s NYC) that have been recently revived (Kim). We are also working to define protein antigens that can be used as imaging agents, therapeutics and diagnostics in point of care devices (Freiberg). I’ll present the above topics from the perspective of how mass spectrometry is helping to solve these problems. I’ll also mention how we are working to get down to single cell analysis for proteomics and lipidomics (Weke). And I will provide some perspective on our capabilities at the Genome BC Proteomic Centre where we carry out fee-for-service experiments in metabolomics and proteomics.

Sandy Markey, Ph.D.

Sanford P. Markey, Ph.D. 1942-2022.

It is with great sadness that we share the news of the death of Sandy Markey, loving husband of Carol, father of Amy (and Shane), Daniel (and Robyn), and grandfather of Ella, Zachary, and Chloe. Sandy lost his long battle with cancer on the morning of February 6, 2022.

All who met Sandy immediately felt his warmth, sincerely, and empathy and shortly afterwards discovered his clarity of thought. These qualities had a major effect on our programs at NIST both before and after his retirement from NIH nearly ten years ago. We develop reference mass spectral libraries here, but even before such a library existed, in the earliest days of the ASMS Sandy lobbied for that that organization to lead a community-based effort to construct such a resource. This reflected his long-held commitment to service and his view of science as a community-based endeavor. This idea of a library was pursued through Hank Fales at NIH, eventually leading to our program – now the NIST/EPA/NIH library, for which he played a role in throughout his career, submitting some of its earliest entries. When I visited Sandy about 20 years ago, he suggested that we build a library of peptide spectra. “Interesting idea” I said, “but what is a peptide?”. He told me and after further instruction we now have a highly developed peptide ion fragmentation library and major involvement in the field of proteomics – including current studies on the SARS-CoV-2 Spike protein and our long involvement with the NIH/NCI Clinical Proteomic Tumor Analysis Consortium program, in which Sandy played a major role. In the meantime, he co-founded the US HUPO organization using creative financing to get it started which he never divulged to me. While at NIH he sent two colleagues to NIST, Jeri Roth and Sara Yang, one of whom managed our initial proteomics program and another who still leads our tandem library software development work. With retirement from NIH in view, they convinced him to accept a 3-days-a-week appointment at NIST. Since then, Sandy has been a central figure in our quality control program and was well known here for his ready assistance to all, especially junior staff, in introducing them to the ways of doing and publishing research. He even advised us of a major animal milk resource at the National Zoo, leading to new libraries and an article in the Washington Post. I am pleased also that we linked him to one of our external evaluators, with whom his enjoyed sailing just a few years ago in, of all places, Tennessee. He was always deeply supportive of members of our group, ready to help them make the best decision. Many members of our group and in the community sorely miss him. His presence here was also a major factor in our recent recruitment of two senior NIH staff who knew him well, Lewis Geer and Douglas Slotta who had hoped to work with him for a much longer time. Sandy has left a deep imprint on the work on many at NIST and in our memories. It is great fortune to have known him. – Stephen Stein, NIST

When I think of Sandy, I think of someone who was always drawing people in. And Sandy was involved in lots of things and knew lots of people. Most recently, we have him to thank for being the motivating force behind incorporating the WBMSDG as a non-profit entity. Sandy drew on his experience and connections in founding and incorporating US HUPO to help us and guide us through the process. Even though that was recent, it feels like it was ages ago. Sandy always made it a point to be very approachable and to know everyone and to be very encouraging. – Jim Kelley, NIH

Sandy was a great mentor for the mass spectrometry community at NIH over many decades. I personally had the benefit of his presence by consulting with him at almost every step of my career advancement. He was very respected and loved by colleagues at NIH. He organized proteomics symposium at NIH in 2001 to stimulate the interest of NIH on the then emerging field. He always promoted the collaborative spirit in the community. At the 2016 symposium held in honor of his retirement from NIH, Sandy urged us to continue the symposium at a regular basis as a new collaborative avenue among mass spectrometry community in the area. He was a warm and caring person, always thinking of the community ahead of his own interest. We will miss him greatly. – Hee-Yong Kim, NIH

Sandy co-organized the WBMSDG in the late 1970s was a speaker in 2013, 2012, 2011, 1990, 1985, and 1982.

Bruker Virtual timsTOF Seminar

New Developments in PASEF & 4D-Proteomics™ on the timsTOF Pro 2 and timsTOF SCP

Register here for this informative, new technology, Mass Spectrometry seminar, Tuesday, February 8th from 2:00pm – 3:00pm EST

• This presentation will highlight the latest proteomics applications data from the timsTOF technology.
• We will show how the extra dimension of separation provided by the mobility separation on the timsTOF enables greater depth from less sample and short acquisition times.
• We will highlight the performance of the timsTOF SCP for single cell proteomics and ultra-high sensitivity applications.
• Example DDA, DIA and PRM workflows will be presented.

Tuesday, February 8th, 2022
2:00 pm – 3:00 pm EST
Speaker: Matt Willetts, Ph.D.
NIH/NIA Host: Andy Y. Qi, Ph.D.
For additional information, please contact Ryan Marsico, Technical Sales Representative for Bruker Daltonics.

January 2022 Virtual Meeting

Speaker: Ricardo Arevalo, University of Maryland

Topic: Laser desorption mass spectrometry with an Orbitrap for planetary exploration

Date: Monday, January 10, 2022

Time: 6:45 Virtual Social, 7:15 pm Presentation

Location: See Zoom invite in email on January 6

Abstract: Laser desorption mass spectrometry (LDMS) techniques enable spatially-resolved chemical analysis of planetary materials, including major/minor/trace element abundances and organic inventory. In the search for prospective biomarkers, the Mars Organic Molecule Analyzer (MOMA) onboard the ExoMars Rosalind Franklin rover will be the first LDMS instrument to characterize the composition of another planet in situ. Here, we describe a next-generation LDMS instrument that integrates a pulsed laser source capable of active beam scanning and precisely-controlled attenuation, and an Orbitrap mass analyzer that delivers 100× higher mass resolution and mass accuracy compared to legacy sensors. A partnership between the University of Maryland, NASA GSFC, the French CosmOrbitrap Consortium, and Thermo Scientific has enabled the development of an engineering test unit that meets the form, fit, and function of a flight instrument targeting the surfaces of Europa, Enceladus, and the Moon.