Speaker: Ling Hao, George Washington University
Topic: Capturing Organelle Dynamics with Multifaceted MS-Omics Strategies
Date: Monday, September 19, 2022
Time: 6:00 pm Dinner and Vendor Night, 7:15 pm Presentation
Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
This will be an in-person meeting. Attendees are required to show a vaccine card (either at the door or in advance using the web form).
Dinner: Please RSVP to Andy Qi (firstname.lastname@example.org) by Friday, September 16th if you will be attending the dinner.
Abstract: Lysosomes and mitochondria are membrane-bound organelles in the cell that are responsible for trash-disposal and ATP production, respectively. Lysosomal and mitochondrial dysfunctions have been linked to numerous human diseases, such as neurodegeneration, cancer, and cardiovascular diseases. These organelles are highly dynamic and frequently interact with other cellular components in a transient fashion, which are difficult to capture with traditional immunoprecipitation and organelle isolation methods. Here, I will describe our recent efforts in developing proximity labeling proteomics, metabolomics, and dynamic SILAC approaches to characterize sub-organelle microenvironment and protein turnover in human iPSC-derived neurons. I will also
introduce our newly established universal and sample type-specific contaminant libraries that can benefit both DDA and DIA proteomics for the broad bottom-up proteomics community.