Speaker: John Janiszewski, NIH/NCATS

Topic: An Open Port Sampling interface for High-throughput Mass Spectrometry:
Emerging Biotechnology for Screening and Reaction Optimization in Drug Discovery

Date: Monday, May 16, 2022

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
This will be an in-person meeting. Attendees are required to show a vaccine card (either at the door or in advance using the web form) . If you have submitted your vaccine card before, your status is already recorded.

Dinner: Please RSVP to Dapeng Chen (cdpumd@gmail.com) by Friday, May 13th if you will be attending the dinner.

Abstract: The Open-Port Sampling Interface (OPI or OPSI) presents a flowing solvent stream that can be adapted for direct sampling by mass spectrometry in a variety of formats. In Acoustic-Droplet ejection Mass spectrometry (ADE-MS) the OPI is positioned directly above an acoustic transducer such that nL sample volumes can be ‘ejected’ from 96- or 384-well plates directly into the OPI flow stream for direct ESI-MS/MS quantitative sampling. AE-MS has been applied in a variety of reaction screening applications in drug discovery. We report on our testing of the OPI in two sampling configurations. An AE-MS application that uses a modified EDC ATS-100 acoustic dispenser for High-Throughput screening of a biochemical (TMPRSS2) enzyme assay, and in vertical orientation for direct droplet sampling using a pneumatic aspiration-dispense pumping system. The pneumatic system delivers a pressure-pulse that can eject a 3µL drop cleanly and consistently (6% CV) for direct OPSI-MS sampling.