Speaker: Yansheng Liu, Yale University School of Medicine
Topic: DIA-MS and Its Application to Profiling Cellular Proteome and Proteoform Dynamics
Date: Monday, November 16th, 2020
Time: 2:00 pm Presentation
Location: Webinar – see emails on Nov. 5 and 12 for invite link. Join the mailing list
Abstract: The term ‘proteoform’ is now used to designate different molecular forms in which the protein product of a single gene can be found, including changes due to genetic variations, alternatively spliced RNA transcripts, and post-translational modifications. Although proteoform is normally studied by the top-down approach, we will discuss a new bottom-up strategy to investigate the site-specific modiforms. We will first review the data-independent acquisition mass spectrometry (DIA-MS) and its development in our group. We will then present how we use DIA-MS, pulse stable isotope-labeled amino acids in cells (pSILAC) approach, and genome-wide correlation analysis for quantifying both abundance and turnover rate of proteins in cancer cell models.