February 2017 Meeting

Speaker: Nathan J. Edwards, Georgetown University

Topic: Beyond Peptide Identification Informatics: Multi-Search, Systems Proteomics, Proteogenomics, Phyloproteomics, and Glycoproteomics

Date: Monday, February 27, 2017

Time: 6:15 pm Dinner, 7:15 pm: Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Katherine Fiedler (Katherine.L.Fiedler@fda.hhs.gov) before February 27 if you will be attending the dinner or are a presenting as a vendor.

Abstract: Bottom-up proteomics by LC-MS/MS is one of the most widely used analytical workflows for characterizing the expressed proteins of the cell. With continued improvements in mass spectrometer speed, accuracy, and versatility, we can increase the depth of coverage and detail of protein characterization, but only if our data analysis capabilities keep pace with the size, and complexity, of the collected spectral data. Tens of spectral datafiles with tens of thousands of spectra are now passé. Protein sequence databases provide multiple proteoforms and amino-acid variants per gene, and the number of species and strains with genome sequences continues to grow. Connecting identified proteins with their pathways, to provide a functional context for differentially abundant proteins remains a challenge, and the exploration of non-template driven post-translational modifications, such as glycosylation, requires novel analytical and mass-spectrometry techniques that demand new data-analysis techniques. Crucially, each of these analytical contexts requires a careful consideration of the potential for false conclusions, and strategies for estimating statistical significance.