Category Archives: Other Training Opportunities

December 15, 2008 WBMSDG Meeting

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Speaker: Dr. Justin Hettick; CDC NIOSH in Morgantown

Topic: Discrimination of microorganisms by MALDI-TOF MS

Abstract: Unambiguous identification of microorganisms such as bacteria or fungi is essential for effective surveillance and treatment of disease.  Unfortunately, diagnosis by classical microbiological methods often requires lengthy culture procedures or subjective micro-and macroscopic evaluations.  To stem the spread of disease, rapid procedures for the accurate identification of microorganisms are necessary.  To that end, we have been developing mass spectrometry-based tools for the analysis of bacteria and fungi.

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOF-MS) can be used to generate highly reproducible, characteristic “fingerprint” mass spectra.  These “fingerprint” mass spectra may be reduced to a subset of significant mass-to-charge values through statistical tools and compared against a reference library.  Using this technique, microorganisms can be easily identified at the species and strain level with error rates approaching zero.

Time: 7:30 pm

Location: Shimadzu Scientific Instruments, Inc. Training Center, 7100 Riverwood Drive, Columbia, MD 21046

Directions: Shimadzu Scientific Instruments

Mass Spec in BioTech Workshop

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Date: October 20-22, 2008.
Location: UMBC
Contact: Alexei Gapeev, Ph.D.; University of Maryland, Baltimore County Dept. of Chemistry and Biochemistry 1000 Hilltop Circle Baltimore, MD 21250 office (410)455-6251 fax (410)455-2608 gapeev@umbc.edu

UMBC mass spectrometry facility is hosting a workshop on mass spectrometry tools for proteomics. The workshop will be held October 20-22 at the main UMBC campus. It will center on the facility nanoLC-MS system. The participants will learn underlying theory of mass spectrometry, liquid chromatography and protein database searching approaches. Hands-on training on the instrument (ThermoFisher LTQ) operation will supplement theory.

Expected outcome: The main goal of the workshop is to provide enough knowledge and hands-on experience so that, upon completion, attendees are able to independently plan experiments, prepare adequate samples, use the instrument in a productive way, recognize problems, conduct database search and interpret experimental results.

Who should attend: Prospective users of the recently installed nano-LC-MS system. Those interested in learning the mass spectrometry based proteomics. The workshop agenda is below: Day 1. Morning (9:30-12 am) and afternoon (1:30-2:30 pm): Instrument operation demonstration. Lecture. Nano-LC front end operation. Introduction to mass spectrometry as applied to the instrument and the discipline of proteomics. Day 1. Afternoon (2:30-4:30 pm):Hands-on exercise: Setting LC protocol and simple MS run. Setting up the instrument and software options. Setting up and running sequences. Day 2. Morning (9:30-12 am): Lecture. Topics include: Ion formation in the ESI source. Ion transmission and detection basics. MS scan modes (full scan, MS/MS, data dependent) and their utilization in mass spectrometry experiments. Protein ID workflow. Day 2. Afternoon: Hands-on exercise. Setting up a data dependent run. Data analysis software: Using software to display data, make annotations, manipulate other display options (e.g. ranges, background subtraction), export to other applications. Day 3. Hands on exercise. Database searching using BioWorks (Sequest) to mine for proteomics information. This section will include the sequest process overview, topics on obtaining a database, creating a database subset, indexing a database to speed up a search, setting specific parameters, interpreting the search data. The participants will analyze data acquired in day 2 and correlate data quality with the methods used.

Bruker Daltronics 2007 Mass Spectrometry Seminars

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New Mass Spectrometry Based Methods for Intact Protein and
Peptide Characterization in Biomarker Research

You are invited to discover how mass spectrometry is advancing the state of the art in protein function and biomarker research. Electrospray Ionization (ESI) and Matrix Assisted Laser Desorption Ionization (MALDI) mass spectrometry are the basis of important new tools that facilitate the characterization of peptides and proteins with high specificity. Complementary techniques are gaining in popularity for biomarker research and discoveries using MALDI imaging and novel ESI-MS/MS fragmentation methods.
Join our seminars to find out more about these emerging new technologies.

For more detail view the attached PDF. bdal_seminars20071

Visit www.brukerseminars.com for more information and registration.

Seminar Series 1
February 6 (9-12 AM): Bethesda, MD
February 7 (9-12 AM): Wilmington, DE
February 8 (9-12 AM): Research Triangle Park, NC

Seminar Series 2
February 20 (12-4 PM): New York City, NY
February 21 (9-12 PM): West Orange, NJ
February 21 (3-6 PM): E. Brunswick, NJ
February 22 (9-12 PM): King of Prussia, PA

The 2nd Workshop on Harsh-Enviroment Mass Spectrometry

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MARCH 18-21, 2001
ST. PETERSBURG, FL
PRESENTED BY: CENTER OF OCEAN TECHNOLOGY UNIVERSITY OF SOUTH FLORIDA

Purpose: In-situ mass spectrometry in a wide variety of harsh environments—from outer space to the earth’s oceans to battlefield scenarios—is rapidly becoming a reality. There are many common features to MS deployment in these vastly different conditions, including high reliability, small size and low power
requirements.

The purpose of this workshop is to promote and encourage interaction among people working on the
deployment of mass spectrometers in different harsh environments, by providing an informal forum for
open discussions and exchange of ideas in a not-so harsh environment.

For more information view this pdf. hemsflyer1