Speaker: Stephanie Cologna, University of Illinois Chicago

Topic: Mass Spectrometry Enabled Proteomics, Lipidomics and Imaging in Niemann-Pick Type C Disease

Date: Monday, October 17, 2022

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
This will be an in-person meeting. Attendees are required to show a vaccine card (either at the door or in advance using the web form) . If you have submitted your vaccine card before, your status is already recorded.

Dinner: Please RSVP to Andy Qi (andy.yue.qi@gmail.com) by Friday, October 14 if you will be attending the dinner.

Abstract: Mass spectrometry-based proteomics and lipidomics are powerful strategies to understand molecular mechanisms associated with human disease. Our laboratory studies Niemann-Pick Type C (NPC), a fatal, progressive neurodegenerative disorder that arises due to improper trafficking of cholesterol. As a result of the genetic defects in NPC, cholesterol storage is observed in late endosome/lysosome compartments. Subsequently, a series of downstream events occur including neuroinflammation, calcium imbalance, oxidative stress and progressive neuron loss with the disorder being ultimately fatal in the early adulthood years. In an effort to understand pathways associated with the downstream consequences of the genetic cause of NPC, we have employed mass
spectrometry imaging, quantitative proteomics and lipidomics in multiple models of NPC. In this seminar, I will share several examples of integrated ‘omics approach to reveal markers of NPC disease as well as seek insight into cell death in NPC using mass spectrometry.

Speaker: Ling Hao, George Washington University

Topic: Capturing Organelle Dynamics with Multifaceted MS-Omics Strategies

Date: Monday, September 19, 2022

Time: 6:00 pm Dinner and Vendor Night, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
This will be an in-person meeting. Attendees are required to show a vaccine card (either at the door or in advance using the web form).

Dinner: Please RSVP to Andy Qi (andy.yue.qi@gmail.com) by Friday, September 16th if you will be attending the dinner.

Abstract: Lysosomes and mitochondria are membrane-bound organelles in the cell that are responsible for trash-disposal and ATP production, respectively. Lysosomal and mitochondrial dysfunctions have been linked to numerous human diseases, such as neurodegeneration, cancer, and cardiovascular diseases. These organelles are highly dynamic and frequently interact with other cellular components in a transient fashion, which are difficult to capture with traditional immunoprecipitation and organelle isolation methods. Here, I will describe our recent efforts in developing proximity labeling proteomics, metabolomics, and dynamic SILAC approaches to characterize sub-organelle microenvironment and protein turnover in human iPSC-derived neurons. I will also
introduce our newly established universal and sample type-specific contaminant libraries that can benefit both DDA and DIA proteomics for the broad bottom-up proteomics community.

Topic: Post-ASMS Poster Night and ASMS Travel Award Presentations

Date: Monday, June 27, 2022

Time: 6:15 pm Dinner (outdoors) and ASMS posters, 7:30 pm Presentations

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
This will be an in-person meeting. Attendees are required to show a vaccine card (either at the door or in advance using the web form).

Dinner: Please RSVP to Dapeng Chen (cdpumd@gmail.com) by Friday, June 24th if you will be attending the dinner and/or if you will participate in the poster session.

ASMS Travel Award Recipients:

• • Haorong Li, George Washington University
    

: “Integrated Proteomics and Metabolomics of Mitochondrial Neurodegenerative Disease MELAS using Patient-derived Fibroblasts”

• • Hannah Lawther, Smithsonian Museum Conservation Institute
    

: “Minimally-Invasive Liquid Microjunction Sampling to Identify Dye on a 17th Century Spanish Illuminated Manuscript”

• • Jie Li, University of Maryland

: “Proteo-Metabolomics of Spemann’s Organizer in the Vertebrate (Frog) Embryo” and “Cell-lineage Based Multi-omics in the Vertebrate (Frog) Embryo”

Speaker: John Janiszewski, NIH/NCATS

Topic: An Open Port Sampling interface for High-throughput Mass Spectrometry:
Emerging Biotechnology for Screening and Reaction Optimization in Drug Discovery

Date: Monday, May 16, 2022

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
This will be an in-person meeting. Attendees are required to show a vaccine card (either at the door or in advance using the web form) . If you have submitted your vaccine card before, your status is already recorded.

Dinner: Please RSVP to Dapeng Chen (cdpumd@gmail.com) by Friday, May 13th if you will be attending the dinner.

Abstract: The Open-Port Sampling Interface (OPI or OPSI) presents a flowing solvent stream that can be adapted for direct sampling by mass spectrometry in a variety of formats. In Acoustic-Droplet ejection Mass spectrometry (ADE-MS) the OPI is positioned directly above an acoustic transducer such that nL sample volumes can be ‘ejected’ from 96- or 384-well plates directly into the OPI flow stream for direct ESI-MS/MS quantitative sampling. AE-MS has been applied in a variety of reaction screening applications in drug discovery. We report on our testing of the OPI in two sampling configurations. An AE-MS application that uses a modified EDC ATS-100 acoustic dispenser for High-Throughput screening of a biochemical (TMPRSS2) enzyme assay, and in vertical orientation for direct droplet sampling using a pneumatic aspiration-dispense pumping system. The pneumatic system delivers a pressure-pulse that can eject a 3µL drop cleanly and consistently (6% CV) for direct OPSI-MS sampling.

Speaker: Glen P. Jackson, West Virginia University

Topic: Hair Reveals what People Conceal: Biometric Traits from the Chemical Analysis of Human Hair

Date: Monday, April 18, 2022

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
This will be an in-person meeting. Attendees are required to show a vaccine card (either at the door or in advance using the web form) . If you have submitted your vaccine card before, your status is already recorded.

Dinner: Please RSVP to Dapeng Chen (cdpumd@gmail.com) by Friday, April 15th if you will be attending the dinner.

Abstract: This presentation reveals the profound ways in which isotope ratio mass spectrometry (IRMS) can provide information about the geographic origin and history of almost everything around us, including our fellow humans. The presentation explains, from an atomic/isotopic perspective, the ways in which we are literally what we eat. The talk also explains how researchers use IRMS measurements of hair to determine the geographic origin and travel histories of animals and humans.
Building upon these established capabilities, our research develops the ability to objectively classify the hair of different human donors into soft biometric grouping factors such as biological sex and alcohol consumption or to predict continuous variables like age. Classification is accomplished either through the quantitation of amino acids in the shafts of human hair, which is comprised mainly of structural proteins named keratins, or through the precise measurement of naturally occurring stable isotopes of 13C/12C of each amino acid in the hair shafts. Our work also demonstrates that amino-acid-specific analysis of human hair can predict type II diabetes in the donors. In other, unrelated applications, we use the same methodology to link individual blowflies to their carrion (flesh) diet and to link Eastern oysters to their harvesting areas.