Memorial Symposium in Honor of Dr. Andrew J. Alpert

Sept. 13-15, 2023, Zoom

It is with heavy hearts and profound respect that we announce this memorial symposium to honor the life and legacy of the esteemed Dr. Andrew J. Alpert. Dr. Alpert is a brilliant scientist, a dedicated mentor, a devoted collaborator, and a legend in the field of chromatography. Dr. Alpert’s significant contributions left an indelible mark on the world of chromatography! Andy is a very kind person! Andy touched the lives of so many, he was always willing to stop what he was doing and help others. Dr. Alpert was also a talented artist, linguist (four languages), musician (pianist), sportsman (pole vaulter), chef, naturalist, and above all – a beloved husband, father, brother, and grandfather to five grandchildren.

The symposium will feature distinguished speakers, colleagues, and friends together with his family members to celebrate his legacy by highlighting the extraordinary contributions he made to the field of chromatography and mass spectrometry-based proteomics as well as the personal connections he forged throughout his life. In addition to the speakers, there will be opportunities for attendees to participate in the virtual receptions and pay their respects through heartfelt tributes. The symposium aims to foster an atmosphere of intellectual exchange, inspiration, and remembrance, just as Dr. Alpert did throughout his distinguished career.

The conference website is now available: https://labs.wisc.edu/aams/. Please register at your earliest convenience. Registration is free but required. Everyone is welcome. Let us come together to celebrate the life and legacy of Dr. Andrew J. Alpert!

With deepest respect,
Organizers (CACA, PolyLC, Inc, 仪器信息网, and Ge Research Group)

September 2023 Meeting and Vendor Night

Speaker: John R. Yates, The Scripps Research Institute

Topic: How a single mutation in CFTR causes the systemic disease cystic fibrosis: interactions, PTMs, and structure

Date: Monday, September 11, 2023

Time: 6:00 pm Dinner and Vendor Night, 7:15 pm Presentation

Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions) This will be an in-person meeting.

Dinner: Please RSVP to Jonathan Ferguson (jonathan.ferguson33@gmail.com) by Friday, September 8 if you will be attending the dinner.

Abstract: Protein conformation is dynamic as it is influenced by post-translational modifications (PTMs) and interactions with other proteins, small molecules or RNA, for example. However, in vivo characterization of protein structures and protein structural changes after perturbation is a major challenge. Therefore, experiments to characterize protein structures are typically performed in vitro and with highly purified proteins or protein
complexes, revealing a static picture of the protein. To identify the true conformational space occupied by proteins in vivo, we developed a novel low-resolution method named Covalent Protein Painting (CPP) that allows the characterization of protein conformations in vivo. Here, we report how an ion channel, the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR), is conformationally changed during biogenesis and channel opening in the cell. Our study led to the identification of a novel opening mechanism for CFTR by revealing that the interaction of the intracellular loop 2 (ICL2) with the nucleotide binding domain 2 (NDB2) of CFTR is needed for channel gating, and this interaction occurs concomitantly with changes to the narrow part of the pore and the walker A lysine in NBD1 for wt CFTR. However, the ICL2:NBD2 interface, which forms a “ball-in-a-socket” motif, is uncoupled during biogenesis, likely to prevent inadvertent channel activation during transport. Mutation of K273 in the ICL2 loop severely impaired CFTR biogenesis and led to accumulation of CFTR in the Golgi and TGN. CPP further revealed that, even upon treatment with current approved drugs such as Trikafta or at permissive temperature, the uncoupled state of ICL2 is a prominent feature of the misfolded CFTR mutants ∆F508 and N1303K that cause Cystic Fibrosis (CF). Although Trikafta treatment reduced the amount of uncoupled ICL2:NBD2 interfaces, more than 75% of F508 CFTR remained in the uncoupled state, suggesting that stabilization of this interface could produce a more efficient CF drug. CPP can characterize a protein in its native environment and measure the effect of complex PTMs and protein interactions on protein structure, making it broadly applicable and valuable for the development of new therapies.
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The Washington-Baltimore Mass Spectrometry Discussion Group is pleased to announce that our first event of 2023-2024 will be kicked off on September 11, 2023 (Monday) at Shimadzu Scientific in Columbia, MD. It is our true privilege this year that a giant in our field, Dr. John Yates III from Scripps Research, will be speaking as the keynote speaker at this event.

Our WBMSDG board has decided to include two lightning talks before the main talk starting with our October event. Each lightning talk lasts 7 minutes with no more than 5 slides, and 5 minutes for Q&A for both talks. The desired speakers are early-career researchers/scientists in mass spec-related areas. They can be junior scientists, postdoctoral fellows, graduate or undergraduate students.

Please submit abstracts to co-chairs to apply for the lightning talks, and our board members will review the abstracts on a rolling basis.

Structure the abstract (maximum 300 words) with the following headings:
Title (maximum 20 words)
Authors and affiliations (Including senior authors/PIs)
Introduction
Methods
Results
Conclusions

(We are updating our sponsors list for 2023-2024.)

2nd International Top-Down Proteomics Symposium

The Consortium for Top-Down Proteomics is very pleased to invite you to the 2nd International Top-Down Proteomics Symposium. The program, hosted by Northwestern University, will take place in Chicago, Illinois, on the University’s beautiful downtown campus on October 3-5, 2023.

View the Program, Registration, and other details at https://tdp2023.topdownproteomics.org/.

Don’t miss this opportunity to learn about exciting advances that are shaping the field of top-down proteomics and participate in discussions about new technologies and approaches that are transforming human biology.

Merger with Washington Chromatography Discussion Group

The Washington Chromatography Discussion Group is merging into the Washington-Baltimore Mass Spectrometry Discussion Group in 2023. You can read more about the Chromatography Group on the History page.

The Washington Chromatography Discussion Group (WCDG) began in the early 1960s as an informal group of gas chromatographers who gathered to exchange ideas related to separation science. In 1965, the group, comprised of distinguished scientists from government, academia, and industry, formally became known as the “Washington Area Gas Chromatography Discussion Group”. The first president was M. Beroza, and president-elect and program chairperson was I. Hornstein, both from the USDA. The group met once a month from September through June in several locations throughout the area to hear an invited speaker and share information. As the group gained popularity, its activities included seminars, dinners, short courses, and symposia. By 1970, HPLC had become a viable technique so the group’s name was changed to the more encompassing “Washington Chromatography Discussion Group.” The group’s meetings were held regularly at one location, the office of Hewlett-Packard in Rockville, Maryland, and instrument manufacturers began sponsoring a light dinner before each meeting. Meetings were later held at the U.S. Pharmacopeia in Rockville and then at the Institute for Bioscience and Biotechnology Research in Rockville.