Speaker: Dr. John Cipollo, FDA/CBER
Topic: Understanding carbohydrate dependent host-pathogen interactions in the surrogate host Caenorhabditis elegans: A mass spectrometry based approach
Date: Monday, May 14, 2012
Time: 7:30 pm
Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
Dinner: Please join the speaker and the co-chairs for dinner at the Ram’s Head Tavern at Savage Mill, 8600 Foundry Street, Savage, MD 20763 at 5:30 pm. Contact Shelley Jackson (Shelley.Jackson@nih.hhs.gov) to let him know you will be there for dinner.
Abstract: Caenorhabditis elegans is infected by over 40 microbial pathogens most of which are human pathogens or their close relatives. Genetic and biochemical evidence suggests that some of these pathogenic interactions are carbohydrate dependent. Here we use a mass spectrometry based approach to investigate the nature of this carbohydrate dependence through comparison of wild type infection susceptible nematode strains and carbohydrate deficient infection resistant strains. The production and use of chemical probes including saccharide loaded latex beads and 2 types of affinity glycoproteomics reagents will be discussed. Also to be discussed will be our efforts towards construction of C. elegans native glycan arrays.