Wednesday, March 17 MSDG Meeting (Joint meeting with Washington Chromatography Discussion Group)

Topic:  Chromatography and Mass Spectrometry of Intact Proteins

Speaker: Dr. Steven M. Musser,  Director, Office of Regulatory Science, Center for Food Safety and Applied Nutrition/FDA, College Park, MD
Time: 7:30 pm; Social Hour: 6:30 pm
Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046

Click here for Meeting Directions

Dinner: Please join co-chairs Alexei Gapeev, Berk Oktem and the speaker for dinner at for dinner at the Ram’s Head Tavern at Savage Mill, 8600 Foundry Street, Savage, MD 20763 at 5:00 pm. Contact Alexei Gapeev (gapeev@umbc.edu ) and let him know you will be there.

Abstract: Although LC/MS of peptides has advanced a great deal, with numerous effective sample preparation techniques, chromatography columns, and automated MS routines, the same can’t be said for the analysis of intact proteins. While peptide analysis is a powerful tool, missed cleavages and incomplete sequence coverage make it difficult to use for the comparison and differentiation of closely related proteins, such as protein toxins, food allergens, and similar bacterial strains. For this reason, we are exploring techniques to effectively extract, separate, and analyze intact proteins, with specific attention to parameters that permit high protein recoveries, reproducible results, and maximize the total number of cellular proteins observed.