Wednesday, June 19th, 2019

March 2019 Meeting – LATE LOCATION CHANGE, SEE BELOW

February 20, 2019 by  
Filed under Meetings, WBMSDG Meetings

Speaker: Liangliang Sun, Michigan State University

Topic: Top-down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry

Date: Monday, March 11, 2019

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Hampton Inn & Suites Columbia, 7045 Minstrel Way, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Yan Wang (yanwang@umd.edu) by Friday, March 8th if you will be attending the dinner.

Abstract: Genome-level and transcriptome-level information cannot accurately reflect proteome-level information because post-transcriptional regulations modulate gene expression,because protein post-translational modifications (PTMs) influence protein function, and because most proteins in cells function as complexes with proteins, RNAs, metals or other small molecules. Characterization of the proteome is imperative to understand the roles played by proteins, protein PTMs and even protein complexes in development and diseases.

Top-down proteomics is a well-known strategy for large-scale characterization of proteome at the intact protein level and is very useful for high-resolution characterization of proteoforms that represent all kinds of protein molecules derived from the same gene due to gene-level variations, RNA-level alternative splicing, and protein-level PTMs. The number of proteoforms in the human proteome has been estimated to be over 1 million. High-capacity separation of proteoforms before electrospray ionization-mass spectrometry and tandem mass spectrometry (ESI-MS and MS/MS) is essential for deep and high-resolution top-down proteomics.

The top-down proteomics community has made tremendous efforts in improving liquid chromatography (LC)-MS and MS/MS for top-down proteomics. We argue that capillary zone electrophoresis (CZE)-MS and MS/MS is another powerful tool for top-down proteomics because CZE can approach high-capacity separation of intact proteins and because CZE-MS has shown obviously higher sensitivity than LC-MS for protein detection. In my talk, I will talk about our recent work on boosting CZE-MS and MS/MS for large-scale and highly sensitive top-down proteomics. I will also introduce our work on developing CZE-MS and MS/MS methodologies for native top-down proteomics that aims to characterize the endogenous protein complexes in cells in discovery mode.…

February 2019 Meeting (note location change)

January 15, 2019 by  
Filed under Meetings, WBMSDG Meetings

Speaker: W. Andy Tao, Purdue University

Topic: Analyses of Protein Phosphorylation and Their Applications in Liquid Biopsy

Date: Monday, February 11, 2019

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: University of Maryland College Park, room 1208, Biology-Psychology building, 4066 Campus Drive, College Park, MD 20742 (parking: 200 Regents Dr, College Park, MD 20742) (Directions)

Dinner: Please RSVP to Yan Wang (yanwang@umd.edu) by Friday, February 8th if you will be attending dinner.

Abstract: Protein kinases and their substrates comprise extensive signaling networks that regulate many diverse cellular functions. However, methods and techniques to systematically identify kinases directly responsible for specific phosphorylation events have remained elusive. Here we describe integrated proteomic strategies to dissect kinase networks in high throughput and demonstrate their applications in multiple systems. Our group has introduced a set of chemical tools and proteomics strategies to analyze protein phosphorylation, in particular, to identify direct kinase substrates and upstream kinases. Recently, for the first time, we identified thousands of phosphoproteins isolated from small volumes of plasma samples and quantitatively measured phosphoproteins that are significantly higher in patients diagnosed with breast cancer as compared to healthy controls. Our studies demonstrate that the development of phosphoproteins as disease biomarkers is highly feasible and may transform disease early detection and monitoring.…

CANCELLED FOR WEATHER (January 2019 Meeting)

December 19, 2018 by  
Filed under Meetings, WBMSDG Meetings

Speaker: Asher Newsome, Smithsonian Museum Conservation Institute

Topic: Ambient Sampling and Ionization for Mass Spectrometry of Museum Objects and Materials

Date: Monday, January 14, 2019

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instruments, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Yan Wang (yanwang@umd.edu) by Friday, January 11th if you will be attending the dinner.

Abstract: Over thirty years after the creation of an atmospheric pressure ionization source for mass spectrometry, development of ambient sampling and ionization sources for use without chromatography exploded beginning in 2004. Whether a given technique moves from academia to industry or follows some other path, oftentimes a design originally intended to be a general-purpose analytical MS tool – versatile, based on fundamental principles, relatively open-source – becomes increasingly engineered toward niche applications, particularly the biomedical and defense markets. With some 150 million objects (including living specimens) in its collection that have been selected for conservation and are available for study, the interests of the Smithsonian Institution fill every niche. The versatility, modularity, and throughput of our mass spectrometry systems are of the utmost concern. A varied selection of our recent projects using ambient ionization, direct analysis in real time (DART), and solid phase microextraction (SPME) to analyze ancient, historic, and modern objects is presented, as well as some of our instrumental modifications to accommodate the particular concerns of a museum.…

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