April Meeting
Co-Sponsored with Washington Chromatography Discussion Group (wcdg.squarespace.com)
Speaker: Michael Boyne, Ph.D., Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD
Topic: Modern Analytics for the Analysis of Complex Drug Products: Physicochemical Characterization using MS
Date: Monday, April 15, 2013
Time: Social Hour and Dinner: 6:00-7:00 pm; Presentation: 7:15 pm
Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
Dinner and Social Hour: Please RSVP to Peter Nemes (Peter.Nemes@fda.hhs.gov) if you will be attending the Dinner and Social Hour at the Shimadzu Scientific Instrument Training Center.
Abstract:
Modern analytical methods continue to improve the ability of analysts to sensitively identify and quantify the structure and composition of complex drugs. The increased information content these technologies provide increases the level of assurance of these products’ quality. For this reason, the FDA has investigated the use of information rich analytical techniques to assess the quality of complex drug products including therapeutic proteins, monoclonal antibodies, and naturally derived small molecules. I will present examples of these techniques applied to currently marketed products.
March Meeting
Meeting Postponed
This meeting has been postponed due to expected bad weather; the meeting will be rescheduled for a later date (posted 3/25/2013- 10:51 am).
Speaker: Dr. Plamen Demirev, Ph.D., Johns Hopkins University Applied Physics Laboratory, Laurel, MD
Topic: Mass Spectrometry in Biodefense
Date: Postponed, TBD
Time: Postponed, TBD
Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
Dinner: Please join the speaker and the co-chairs for dinner at the Ram’s Head Tavern at Savage Mill, 8600 Foundry Street, Savage, MD 20763 at 5:30 pm. Contact Peter Nemes (Peter.Nemes@fda.hhs.gov) to let him know you will be there for dinner.
Abstract: Recent applications of mass spectrometry in biodefense will be discussed. These include a MALDI MS system (CB-TOF), and methods and assays for rapid detection of drug-resistant microorganisms or biological toxins (ricin). The CB-TOF system includes a robotic station for rapid sample preparation for MALDI MS, novel detection and matching algorithms, and a user-friendly graphical interface. To determine drug resistance in bacteria, a mass spectrum of an intact microorganism grown in drug-containing stable isotope-labeled media is compared to a spectrum of the intact microorganism grown in non-labeled media. We have also adapted and streamlined functional MS assays for rapid ricin detection in unknown powder materials by monitoring the depurination of selected DNA substrates.
February Meeting
Speaker: Dr. Sanford P. Markey, Ph.D., Laboratory of Neurotoxicology, NIH, Bethesda, MD
Topic: Of Mice and Men: Reflections on Past and Current Research at NIH
Date: Monday, February 11, 2013
Time: 7:30 pm
Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
Dinner: Please join the speaker and the co-chairs for dinner at the Ram’s Head Tavern at Savage Mill, 8600 Foundry Street, Savage, MD 20763 at 5:30 pm. Contact Peter Nemes (Peter.Nemes@fda.hhs.gov) to let him know you will be there for dinner.
Abstract: From the early days of GC-MS when success required mastery of the arts and sciences of glass blowing and derivatization chemistry to the present era of proteomics with high performance LC/MS/MS instrumentation, the capacity of mass spectrometry to provide definitive, unbiased answers has brought intriguing biomedical problems into our labs. I will reflect on some past research, and describe how a new technique, invented by a multi-disciplinary trans-NIH team, is being implemented for high-throughput micro sample procurement. Expression Microdissection (xMD) retrieves subcellular components or discrete cells directly from stained tissue sections in an unsupervised manner, and permits subsequent proteome analyses of the targeted, enriched material.
