Speaker: Liangliang Sun, Michigan State University

Topic: Top-down Proteomics Using Capillary Zone Electrophoresis-Tandem Mass Spectrometry

Date: Monday, March 11, 2019

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Hampton Inn & Suites Columbia, 7045 Minstrel Way, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Yan Wang ( by Friday, March 8th if you will be attending the dinner.

Abstract: Genome-level and transcriptome-level information cannot accurately reflect proteome-level information because post-transcriptional regulations modulate gene expression,because protein post-translational modifications (PTMs) influence protein function, and because most proteins in cells function as complexes with proteins, RNAs, metals or other small molecules. Characterization of the proteome is imperative to understand the roles played by proteins, protein PTMs and even protein complexes in development and diseases.

Top-down proteomics is a well-known strategy for large-scale characterization of proteome at the intact protein level and is very useful for high-resolution characterization of proteoforms that represent all kinds of protein molecules derived from the same gene due to gene-level variations, RNA-level alternative splicing, and protein-level PTMs. The number of proteoforms in the human proteome has been estimated to be over 1 million. High-capacity separation of proteoforms before electrospray ionization-mass spectrometry and tandem mass spectrometry (ESI-MS and MS/MS) is essential for deep and high-resolution top-down proteomics.

The top-down proteomics community has made tremendous efforts in improving liquid chromatography (LC)-MS and MS/MS for top-down proteomics. We argue that capillary zone electrophoresis (CZE)-MS and MS/MS is another powerful tool for top-down proteomics because CZE can approach high-capacity separation of intact proteins and because CZE-MS has shown obviously higher sensitivity than LC-MS for protein detection. In my talk, I will talk about our recent work on boosting CZE-MS and MS/MS for large-scale and highly sensitive top-down proteomics. I will also introduce our work on developing CZE-MS and MS/MS methodologies for native top-down proteomics that aims to characterize the endogenous protein complexes in cells in discovery mode.…

2nd Annual North American Mass Spectrometry Summer School

July 21-24, 2019 Madison, WI

Please join us for our second annual North American Mass Spectrometry Summer School. We are proud to have assembled world-leading experts in mass spectrometry for this two and a half-day course. Students will experience an engaging and inspiring program covering fundamentals of mass spectrometry and its application to biological systems analysis. Tutorial lectures and hands-on workshops for scientific and professional development are planned. This workshop is made possible by generous support from the National Science Foundation (Integrated Organismal Systems, Plant Genome Research Program, Grant No. 1546742), the National Institutes of Health National Center for Quantitative Biology of Complex Systems (P41 GM108538), Thermo Fisher Scientific, and Promega. Applications for Mass Spec Summer School close on March 15, 2019 at midnight.

Expert Instructors:
Josh Coon – University of Wisconsin
Ulrike Kusebauch – Institute for Systems Biology
Jesse Meyer – University of Wisconsin
Doug Sheeley – National Institutes of Health
Evgenia Shishkova – University of Wisconsin
Beatrix Ueberheide – New York University School of Medicine
Judit Villen – University of Washington-Seattle
Mike Westphall – University of Wisconsin
Evan Williams – University of California-Berkeley

Tutorial Lecture Topics:
Data Analysis
Mass Analyzers
Tandem MS
Shotgun Proteomics, Instrument Acquisition, and Setup

Hands-on Workshops:
Data Integration
Mass Spec 101
Intellectual Property
Science Writing
Scientific Illustrations
Spectral Interpretation
NIH funding and grant process

Organizing Committee: Evgenia Shishkova, Laura Van Toll, and Josh Coon…

February 2019 Meeting (note location change)

Speaker: W. Andy Tao, Purdue University

Topic: Analyses of Protein Phosphorylation and Their Applications in Liquid Biopsy

Date: Monday, February 11, 2019

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: University of Maryland College Park, room 1208, Biology-Psychology building, 4066 Campus Drive, College Park, MD 20742 (parking: 200 Regents Dr, College Park, MD 20742) (Directions)

Dinner: Please RSVP to Yan Wang ( by Friday, February 8th if you will be attending dinner.

Abstract: Protein kinases and their substrates comprise extensive signaling networks that regulate many diverse cellular functions. However, methods and techniques to systematically identify kinases directly responsible for specific phosphorylation events have remained elusive. Here we describe integrated proteomic strategies to dissect kinase networks in high throughput and demonstrate their applications in multiple systems. Our group has introduced a set of chemical tools and proteomics strategies to analyze protein phosphorylation, in particular, to identify direct kinase substrates and upstream kinases. Recently, for the first time, we identified thousands of phosphoproteins isolated from small volumes of plasma samples and quantitatively measured phosphoproteins that are significantly higher in patients diagnosed with breast cancer as compared to healthy controls. Our studies demonstrate that the development of phosphoproteins as disease biomarkers is highly feasible and may transform disease early detection and monitoring.…