Speaker: Renã Robinson, Vanderbilt University
Topic: Enhanced Multiplexing Proteomics to Study Alzheimer’s Disease
Date: Monday, May 21, 2018
Time: 6:15 pm Dinner, 7:15 pm Presentation
Location: Shimadzu Scientific Instrument, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)
Dinner: Please RSVP to Stefani Thomas (firstname.lastname@example.org) before Friday, May 18th if you will be attending the dinner.
Abstract: Sampling changes across multiple tissues in disease can inform of system wide biology for disease understanding and potential biomarker detection. In Alzheimer’s disease, peripheral tissues are recognized as having significant contributions to this brain disorder and in offering biomarkers of disease. Our laboratory is developing proteomics technology to understand these contributions using a mouse model of Alzheimer’s disease, in which brain, heart, and liver, amongst other tissues, can be evaluated. Specifically, we have developed a multiplexing assay termed, combined precursor isotopic labeling and isobaric tagging (cPILOT), to sample protein changes across genotype, multiple tissues, and biological replicates in a single experiment. While there are inherent tradeoffs with a dual labeling approach, the benefits gained in cost and time savings and comprehensive quantitative information are extremely valuable for this platform. This presentation will discuss cPILOT and considerations for the utility of cPILOT in enabling studies of Alzheimer’s disease.