September 2020 Virtual Meeting

Speaker: Joe Cannon, Merck

Topic: Simplifying Cyclic Peptide Hydrolysis Interpretation in Metabolite Identification

Date: Monday, September 21st, 2020

Time: 1:00 pm Presentation

Location: Webinar – see emails on Sept. 10 and 18 for invite link. Join the mailing list

Abstract: Peptides, as therapeutics, can be used to disrupt crucial protein-protein interactions in disease states. As a modality, they offer an exciting compromise between large molecule-like specificity and small molecule-like absorption and distribution, all without the need for orthosteric binding. Despite these favorable properties, peptides suffer from short in vivo half-life due to protease mediated hydrolysis. To combat this, peptides are often conjugated and internally crosslinked to enhance rigidity or cyclized to decrease susceptibility to amino and carboxypeptidases. For linear peptides, identifying the site of hydrolysis is simple and similar to peptide identification in proteomics experiments. For cyclized peptides, it is very challenging due to the fact that hydrolysis simply linearizes the molecule, and the mass of a peptide from hydrolysis at one position is isobaric with every other position. 2-pyridine carboxaldehyde (2PCA) is used here to selectively conjugate the N-terminal amino acid and provide an amino acid specific low mass reporter fragment ion doublet that points to the site of hydrolysis. The chemistry is demonstrated here on a HeLa tryptic digest and proof-of-concept studies are shown on cyclic peptides.

June 2020 Virtual Meeting

Topic: Post-ASMS Virtual Poster Night and ASMS Travel Award Presentations

Date: Monday, June 22nd, 2020

Time: Poster listing online; 1:00 pm Presentations (Zoom link in June 11, 19 email)

ASMS Travel Award Recipients:

    • Gessica Vasconcelos, George Washington University

: “Fiber-based laser ablation electrospray ionization mass spectrometry for molecular profiling and metabolite gradients in anatomical regions selected by fluorescence microscopy”

    • Kunyu Zheng, Georgetown University

: “Non-targeted detection of fluorinated compounds using dielectric barrier discharge nano-electrospray ionization”

    • Yulemni Morel, Univ. of Maryland School of Pharmacy

: “Integrating Qual/Quan Workflows for Combined Structure Characterization and Quantitation of Vinyl Ether Phosphatidylethanolamine”

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WBMSDG attendees are invited to submit their posters for an online listing in lieu of an in-person poster night. Submit yours via the form below!

MP 031 Non-proximate Ambient Sampling for Solvent-free Analysis of Intact Objects; G. Asher Newsome1; Kathleen Martin2; Julia Campbell-Such3; 1Smithsonian Museum Conservation Institute, Suitland, MD; 2Smithsonian National Museum of the American Indian, Hillcrest Heights, MD; 3Smithsonian National Museum of African Art, Washington, DC

MP 206 Optimization of a targeted, multi-allergen LC-MS/MS method for the quantification of egg, milk, and peanut in food; Weili Xiong1; Christine H. Parker1; Katherine L. Fiedler1; 1U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, MD

MP 296 The Inelasticity of Ion-Molecule Collisions in Ion Mobility Spectrometry, Glenn Spangler, Technispan LLC

MP 355 Hepatic dyslipidemia in little brown bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus) with white nose syndrome; Evan L. Pannkuk1; Nicole A. S.-Y. Dorville2; Yvoone A. Dzal2; Quinn E. Fletcher2; Kaleigh J. O. Norquay2; Craig K. R. Willis2; Albert J. Fornace Jr.1,3; Evagelia C. Laiakis1,3; 1Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC; 2Department of Biology, University of Winnipeg, Winnipeg, MB; 3Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC

TP 265 Enhanced Ionization and Facile Selection of Ions at Atmospheric-pressure by Perturbation-induced Pulsing of nano-ESI Coupled to a Single Ion Gate; William McMahon and Kaveh Jorabchi; Department of Chemistry, Georgetown University, Washington, DC

TP 516 Measuring protein conformational change in living cells by quantitative, comprehensive, and ultra-sensitive protein footprinting. Jenna G. Caldwell1*, Bjorn-Erik Wulff1, Lichao Zhang2, Joshua E. Elias2, Pehr A.B. Harbury2. 1Department of Biochemistry, Stanford School of Medicine, Stanford, CA; 2Chan Zuckerberg Biohub, Stanford, CA

WP 020 Identification approaches of the efflorescence detected in Infinity of Nations at the Smithsonian National Museum of the American Indian; Alba Alvarez-Martin* [1]; John George [2]; Emily Kaplan [2]; Lauren Osmond [2]; Leah Bright [2]; G. Asher Newsome [1]; Gwénaëlle Kavich [1]; Rachel Kaczkowski [1]; Susan Heald [2]. [1] Museum Conservation Institute, Smithsonian Institution, Suitland, MD; [2] The National Museum of the American Indian, Smithsonian Institution, Washington, DC

WP 462 Mass Spectrometric Approaches for the Structural Characterization of Chemically Targeted PreQ1 Riboswitches; Christopher C. Lai1; Desta D. Bume1; John S. Schneekloth, Jr.1; James A. Kelley1. 1Chemical Biology Laboratory, Center for Cancer Research, NCI-Frederick, National Institutes of Health, Frederick, MD

ThP 279 Trypsin – a Tired Workhorse? The Selectivity of Atypical Cleavages by Trypsin; Meghan Burke1; Yuxue Liang1; Stephen E. Stein1; 1National Institute of Standards and Technology, Gaithersburg, Maryland

ASMS 2020 Reboot

We hope all the WBMSDG members are having virtual fun at the ASMS 2020 Reboot! Remember, all content will be viewable until August 31. Use the hashtags #ASMS2020 and #WBMSDG on social media to highlight research from our community!

Download the WBMSDG virtual background for Zoom meetings

The organizers at ASMS are putting together a Zoom happy hour for the mass spec community and trainees are especially encouraged to participate and socialize! The happy hour caps at 100 participants.
Registration for US/night owls (Jun 8, 2020 05:30 PM to 09:30 PM EDT): (closed)

Waters IMS webinars

Join Waters for a FREE webinar on:

The Revolutionary Cyclic IMS and an Exploration of its Capabilities

Advances in mass spectrometry (MS) made during the past century have helped empower scientists to research applications in the life, chemical and material sciences. It is the challenging problems that researchers face that underpin innovation in high performance
instrumentation.

Presentation I:
Introduction to Ion Mobility and the SELECT SERIES Cyclic IMS
Discover how revolutionary cyclic ion mobility separation is combined with modern, state‐of‐the‐art time‐of‐flight mass spectrometry to overcome traditional limitations and provide new approaches. The novel cyclic ion mobility device provides scalable, high‐resolution ion mobility separations and provides the ability to perform ion mobility/ion mobility and IMSn experiments.
presenter:
Kevin Giles
Scientific Fellow
Waters MS Research
Waters Corporation

Presentation II:
Cyclic IMS – Applications, Benefits and the Future…
Learn how the unique capabilities of Cyclic Ion Mobility Mass Spectrometry are enabling researchers to unlock new information in drug discovery, biomolecular characterization, biomarker research and many other application areas and how users are influencing further development of this system.
presenter:
Emma Marsden-Edwards
Principal Product Manager
Waters Advanced MS Technologies
Waters Corporation

Each presentation is 30 minutes, and at the end of the second presentation there will be a 30 minute Q&A session. Learn more or register

May 2020 Virtual Meeting

Speaker: Ira Lurie, George Washington University

Topic: Is GC-MS still the gold standard for the analysis of seized drugs?

Date: Thursday, May 21st, 2020

NEW TIME: 1:00 pm Presentation

Location: Webinar – see emails on May 12 and 19 for invite link. Join the mailing list

Abstract: Gas chromatography electron impact mass spectrometry (GC-EI MS) has been considered the gold standard for the analysis of seized drugs. This technique which combines a high resolution separation step with a highly selective detection technique is well established for commonly encountered illicit drugs such as cocaine and heroin. Most recently there has been an up surge of the so called designer drugs, structural or functional analogs of controlled substances that have been synthesized to mimic the pharmacological effects of the original drug, therefore avoiding classification as illegal and/or detection in standard drug tests. The analysis of these emerging drugs is complicated using GC-EI MS by the high number of analogues present in a given class, for which there exists structurally very similar compounds, such as positional isomers and stereoisomers. Problems encountered during analysis include co-elution, poor chromatographic performance, lack of molecular or diagnostic ions, indistinguishable mass spectra, and solute thermal instability. In this seminar alternative liquid phase techniques will be presented including ultra high performance liquid chromatography (UHPLC) with time of flight (TOF) MS detection, high performance supercritical fluid chromatography (UHPSFC) with MS and UV detection, and multi-dimensional UHPLC with UV and MS detection. In regard to gas phase analysis, the use of cold EI-MS and vacuum ultraviolet (VUV) detection will be explored including the use of a tandem GC-VUV-MS instrument. Emerging drug classes investigated include synthetic cannabinoids, synthetic cathinones, fentanyl analogues and phenethylamine analogues.