June 2021 Virtual Meeting

Speaker: Bindesh Shrestha, Waters

Topic: Biomedical Applications of MALDI and DESI Imaging using High-Resolution Ion Mobility Mass Spectrometry

Date: Monday, June 21th, 2021

Time: 1:00 PM EDT Presentation

Location: Webinar – see emails on June 10 and 17 for invite link. Join the mailing list

Abstract: In the last decade, both matrix-assisted laser desorption/ionization (MALDI) and desorption electrospray ionization (DESI) imaging mass spectrometry (MS) have been developed to spatially map small molecules, such as metabolites, lipids and drugs, on tissue. DESI often detects a complementary set of molecules to MALDI due to preferential ionization of those species in electrospray. In this presentation, biomedical applications using both imaging modalities, MALDI and DESI, will be shown. Multimodal imaging using both techniques widens the molecular coverage of many small molecules, such as drugs – with some analyte preferentially ionizing more robustly with one ionization technique versus another. Analysis of ions detected by DESI and MALDI on consecutive sections using similar solvent composition eluded to the complementary coverage of the two mechanistically different ionization sources. In addition to showing the benefits of multimodal imaging MS strategy, the advantages of integrating ion mobility separations during the imaging MS process will also be discussed. Ion mobility separation can isolate ions based on their collision cross-section. For example, imaging a specific lipid molecule on tissue is often challenging because of the large structural diversity of similar lipids present in the sample. Multipass ultrahigh-resolution ion mobility separation can improve the specificity of lipid imaging by DESI, allowing us to image isobaric ions separately. All imaging MS experiments were performed on quadrupole time of flight mass spectrometers, such as SYNAPT XS and Cyclic IMS, with traveling wave ion mobility separation and using High Definition Imaging (HDI) software.

May 2021 Virtual Meeting

Speaker: Martin Jarrold, Indiana University

Topic: Charge Detection Mass Spectrometry: Recent Developments and Applications

Date: Monday, May 17th, 2021

Time: 1:00 PM EDT Presentation

Location: Webinar – see emails on May 6 and 13 for invite link. Join the mailing list

Abstract: Charge detection mass spectrometry (CDMS) provides a way to measure accurate masses for heterogeneous samples into the gigadalton regime. It is a single particle technique where the mass of each ion is determined from simultaneous measurements of its mass to charge ratio (m/z) and charge. CDMS opens the door to the analysis of high mass, heterogeneous samples such as vaccines and gene therapy products. Recent developments in instrumentation that have improved the resolution and speed of CDMS measurements will be described along with several recent examples of applications to the analysis of bio-pharmaceuticals, and virus assembly and disassembly.

April 2021 Virtual Meeting

Speaker: Stefani Thomas, University of Minnesota

Topic: Beyond the BRCA genes: A proteome-centric view of high-grade serous ovarian cancer

Date: Monday, April 19th, 2021

Time: 2:00 PM Presentation

Location: Webinar – see emails on April 9 and 15 for invite link. Join the mailing list

Abstract: The mutational status of a solid tumor can predict the therapeutic efficacy of a specific drug in a molecularly defined subset of patients. Poly (ADP-ribose) polymerase inhibitors (PARPi) have emerged as a novel class of drugs to treat advanced ovarian cancer with mutations in BRCA1/2 genes. Unfortunately, there is considerable inter-patient heterogeneity in BRCA1/2–based determinations of PARPi treatment sensitivity. Determining proteome-level mechanisms of PARPi sensitivity could enhance our ability to select the ovarian cancer patient population that would benefit the most from PARPi therapy, consequently improving survival and overall treatment response. Our laboratory is applying mass spectrometry-based proteomics to identify protein signatures of PARPi sensitivity. This presentation will provide an overview of the experimental models and analytical approaches that we are utilizing toward a long-term goal of identifying prognostic protein biomarkers of PARPi sensitivity in patients with high-grade serous ovarian cancer.

3rd Annual North American Mass Spectrometry Summer School

3rd Annual North American Mass Spectrometry Summer School

June 29 – July 1, 2021 Virtual

Join us for our third annual mass spectrometry summer school. We are proud to have assembled over a dozen world leading experts in mass spectrometry for this three-day course. Our goal is to provide our students, both from academia and industry, an engaging and inspiring program covering the latest in the application of mass spectrometry to omic analyses. Tutorial lectures range from mass analyzers to the basics of data analysis. Also planned are several workshops – aimed at both scientific and professional development. This program is made possible by generous funding from the National Science Foundation (Integrated Organismal Systems, Plant Genome Research Program, Grant No. 2010789) and the National Institutes of Health National Center for Quantitative Biology of Complex Systems (P41 GM108538). As such, there is no cost to participate.

Registration closes on May 15, 2021: https://www.ncqbcs.com/resources/training/summer-school

Expert Instructors:
Scott McLuckey | Purdue University
Rachel Loo | University of California-Los Angeles
Joshua Coon | University of Wisconsin-Madison
Shawnna Buttery | STAR Protocols, Cell Press
Jesper Velgaard Olsen | University of Copenhagen
Lingjun Li | University of Wisconsin-Madison
Jürgen Cox | Max Planck Institute of Biochemistry
Edward Huttlin | Harvard University
Susan Olesik | Ohio State University
Evgenia Shishkova | University of Wisconsin-Madison
Jessica Prenni | Colorado State University
Vicki Wysocki | Ohio State University
John Bowden | University of Florida

Tutorial Lectures:
Tandem MS
Separations
Mass analyzers
Ionization
Data acquisition
Quantification
Data analysis
Experimental design
PTMs
Metabolomics
Lipidomics
Top-down/Native MS

Workshops:
Mass analyzers
Data analysis
Telling your science story
Science writing
Science illustrations

March 2021 Virtual Meeting

Speaker: Michael Marty, University of Arizona

Topic: Using native mass spectrometry and nanodiscs to study assembly of membrane protein and antimicrobial peptide complexes

Date: Monday, March 15th, 2021

Time: 1:00 PM Presentation

Location: Webinar – see emails on March 4 and 11 for invite link. Join the mailing list

Abstract: Native mass spectrometry (MS) has emerged as a powerful technique for studying the oligomeric state and interactions of membrane proteins. However, native MS can be challenging for smaller and more fragile membrane protein and transmembrane peptide complexes. To address this challenge, we have used native MS to study small viral membrane protein complexes in both detergent micelles and lipid nanodiscs. Using charge reduction reagents for stabilization and advanced data analysis techniques to unravel complex spectra, we discovered that viroporin complexes can be highly sensitive to their environment, showing different oligomeric states in different lipids/detergents, with different pH conditions, and with addition of antiviral drugs. Unexpected oligomeric states have been observed that do not match existing structural models, highlighting previously unseen behavior of these complexes. We have also studied assembly of antimicrobial peptide complexes in lipid nanodiscs. Antimicrobial peptides are also highly sensitive to the lipid environment, showing unique assembly pathways that are controlled by the thickness of the lipid bilayer. Ultimately, we expect these applications of native MS will reveal new insights into the biology of infectious disease and provide new directions for antiviral and antibacterial drug discovery.