September 2020 Virtual Meeting

Speaker: Joe Cannon, Merck

Topic: Simplifying Cyclic Peptide Hydrolysis Interpretation in Metabolite Identification

Date: Monday, September 21st, 2020

Time: 1:00 pm Presentation

Location: Webinar – see emails on Sept. 10 and 18 for invite link. Join the mailing list

Abstract: Peptides, as therapeutics, can be used to disrupt crucial protein-protein interactions in disease states. As a modality, they offer an exciting compromise between large molecule-like specificity and small molecule-like absorption and distribution, all without the need for orthosteric binding. Despite these favorable properties, peptides suffer from short in vivo half-life due to protease mediated hydrolysis. To combat this, peptides are often conjugated and internally crosslinked to enhance rigidity or cyclized to decrease susceptibility to amino and carboxypeptidases. For linear peptides, identifying the site of hydrolysis is simple and similar to peptide identification in proteomics experiments. For cyclized peptides, it is very challenging due to the fact that hydrolysis simply linearizes the molecule, and the mass of a peptide from hydrolysis at one position is isobaric with every other position. 2-pyridine carboxaldehyde (2PCA) is used here to selectively conjugate the N-terminal amino acid and provide an amino acid specific low mass reporter fragment ion doublet that points to the site of hydrolysis. The chemistry is demonstrated here on a HeLa tryptic digest and proof-of-concept studies are shown on cyclic peptides.

June 2020 Virtual Meeting

Topic: Post-ASMS Virtual Poster Night and ASMS Travel Award Presentations

Date: Monday, June 22nd, 2020

Time: Poster listing online; 1:00 pm Presentations (Zoom link in June 11, 19 email)

ASMS Travel Award Recipients:

    • Gessica Vasconcelos, George Washington University

: “Fiber-based laser ablation electrospray ionization mass spectrometry for molecular profiling and metabolite gradients in anatomical regions selected by fluorescence microscopy”

    • Kunyu Zheng, Georgetown University

: “Non-targeted detection of fluorinated compounds using dielectric barrier discharge nano-electrospray ionization”

    • Yulemni Morel, Univ. of Maryland School of Pharmacy

: “Integrating Qual/Quan Workflows for Combined Structure Characterization and Quantitation of Vinyl Ether Phosphatidylethanolamine”

Download the WBMSDG virtual background for Zoom


WBMSDG attendees are invited to submit their posters for an online listing in lieu of an in-person poster night. Submit yours via the form below!

MP 031 Non-proximate Ambient Sampling for Solvent-free Analysis of Intact Objects; G. Asher Newsome1; Kathleen Martin2; Julia Campbell-Such3; 1Smithsonian Museum Conservation Institute, Suitland, MD; 2Smithsonian National Museum of the American Indian, Hillcrest Heights, MD; 3Smithsonian National Museum of African Art, Washington, DC

MP 206 Optimization of a targeted, multi-allergen LC-MS/MS method for the quantification of egg, milk, and peanut in food; Weili Xiong1; Christine H. Parker1; Katherine L. Fiedler1; 1U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, MD

MP 296 The Inelasticity of Ion-Molecule Collisions in Ion Mobility Spectrometry, Glenn Spangler, Technispan LLC

MP 355 Hepatic dyslipidemia in little brown bats (Myotis lucifugus) and big brown bats (Eptesicus fuscus) with white nose syndrome; Evan L. Pannkuk1; Nicole A. S.-Y. Dorville2; Yvoone A. Dzal2; Quinn E. Fletcher2; Kaleigh J. O. Norquay2; Craig K. R. Willis2; Albert J. Fornace Jr.1,3; Evagelia C. Laiakis1,3; 1Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC; 2Department of Biology, University of Winnipeg, Winnipeg, MB; 3Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC

TP 265 Enhanced Ionization and Facile Selection of Ions at Atmospheric-pressure by Perturbation-induced Pulsing of nano-ESI Coupled to a Single Ion Gate; William McMahon and Kaveh Jorabchi; Department of Chemistry, Georgetown University, Washington, DC

TP 516 Measuring protein conformational change in living cells by quantitative, comprehensive, and ultra-sensitive protein footprinting. Jenna G. Caldwell1*, Bjorn-Erik Wulff1, Lichao Zhang2, Joshua E. Elias2, Pehr A.B. Harbury2. 1Department of Biochemistry, Stanford School of Medicine, Stanford, CA; 2Chan Zuckerberg Biohub, Stanford, CA

WP 020 Identification approaches of the efflorescence detected in Infinity of Nations at the Smithsonian National Museum of the American Indian; Alba Alvarez-Martin* [1]; John George [2]; Emily Kaplan [2]; Lauren Osmond [2]; Leah Bright [2]; G. Asher Newsome [1]; Gwénaëlle Kavich [1]; Rachel Kaczkowski [1]; Susan Heald [2]. [1] Museum Conservation Institute, Smithsonian Institution, Suitland, MD; [2] The National Museum of the American Indian, Smithsonian Institution, Washington, DC

WP 462 Mass Spectrometric Approaches for the Structural Characterization of Chemically Targeted PreQ1 Riboswitches; Christopher C. Lai1; Desta D. Bume1; John S. Schneekloth, Jr.1; James A. Kelley1. 1Chemical Biology Laboratory, Center for Cancer Research, NCI-Frederick, National Institutes of Health, Frederick, MD

ThP 279 Trypsin – a Tired Workhorse? The Selectivity of Atypical Cleavages by Trypsin; Meghan Burke1; Yuxue Liang1; Stephen E. Stein1; 1National Institute of Standards and Technology, Gaithersburg, Maryland

May 2020 Virtual Meeting

Speaker: Ira Lurie, George Washington University

Topic: Is GC-MS still the gold standard for the analysis of seized drugs?

Date: Thursday, May 21st, 2020

NEW TIME: 1:00 pm Presentation

Location: Webinar – see emails on May 12 and 19 for invite link. Join the mailing list

Abstract: Gas chromatography electron impact mass spectrometry (GC-EI MS) has been considered the gold standard for the analysis of seized drugs. This technique which combines a high resolution separation step with a highly selective detection technique is well established for commonly encountered illicit drugs such as cocaine and heroin. Most recently there has been an up surge of the so called designer drugs, structural or functional analogs of controlled substances that have been synthesized to mimic the pharmacological effects of the original drug, therefore avoiding classification as illegal and/or detection in standard drug tests. The analysis of these emerging drugs is complicated using GC-EI MS by the high number of analogues present in a given class, for which there exists structurally very similar compounds, such as positional isomers and stereoisomers. Problems encountered during analysis include co-elution, poor chromatographic performance, lack of molecular or diagnostic ions, indistinguishable mass spectra, and solute thermal instability. In this seminar alternative liquid phase techniques will be presented including ultra high performance liquid chromatography (UHPLC) with time of flight (TOF) MS detection, high performance supercritical fluid chromatography (UHPSFC) with MS and UV detection, and multi-dimensional UHPLC with UV and MS detection. In regard to gas phase analysis, the use of cold EI-MS and vacuum ultraviolet (VUV) detection will be explored including the use of a tandem GC-VUV-MS instrument. Emerging drug classes investigated include synthetic cannabinoids, synthetic cathinones, fentanyl analogues and phenethylamine analogues.

April 2020 Virtual Meeting

Speaker: Ben Neely, NIST

Topic: Sea lions and bats and humans, oh my! How to explore mammalian serum proteomes

Date: Monday, April 20th, 2020

NEW TIME: 1:00 pm Presentation

Location: Webinar – see email on April 16 for invite link. Join the mailing list

Abstract: Comparative biology and biomimicry are broadly focused on understanding the underlying molecular basis of phenotypes relevant to chronic human ailments. Notably, research in hibernating mammals is improving our understanding of neurodegeneration, studies of diving mammals is identifying novel mechanisms of ischemia/reperfusion injury resistance, while studying organisms that follow Peto’s paradox is advancing longevity and cancer research. In addition to biomimetics, recent events are highlighting certain mammal’s ability to serve as reservoirs of infectious disease. Systematically characterizing the diversity of all mammalian proteomes will enable unexpected discoveries, but this presents numerous technological hurdles. In this seminar I will present results, recommendations and solutions to the issues of working in species without genomes or annotations, acquiring proteomic data in a standardized fashion, comparing proteomes between species and identifying molecular trends across clades with relevant phenotypes. Using the Atlantic bottlenose dolphin and California sea lion as examples, I will demonstrate improvements in proteomic analysis using genomic sequencing and gene annotation techniques, as well as emerging proteomic techniques such as data-independent acquisition applied to undepleted human and bat serum.

CANCELLED: March 2020 Meeting

Speaker: Carlos Larriba-Andaluz, Purdue School of Engineering & Technology

Topic: Understanding Ion Mobility Separation in High-Resolution Instruments. Caveats of and deviations from the Mason-Schamp Equation for small molecules.

Look for this talk to be re-scheduled in the future!