December 2019 Meeting

Speaker: Asher Newsome, Smithsonian Institution

Topic: Ambient Sampling and Ionization for Mass Spectrometry of Museum Objects
and Materials

Date: Monday, December 16th, 2019

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instruments, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Meghan Burke (meghan.burke@nist.gov) by Friday, December 13th if you will be attending the dinner.

Abstract: The pace of development of ambient mass spectrometry has hardly slowed since kicking off fifteen years ago. Whether a given technique moves from academia to industry or follows some other path, often a design originally intended to be a general-purpose analytical MS tool – versatile, based on fundamental principles, relatively open-source – becomes increasingly engineered toward niche applications, particularly the biomedical and defense markets. With some 150 million objects (including living specimens) in its collection that have been selected for conservation and are available for study, the interests of the Smithsonian Institution fill every niche. The versatility, modularity, and throughput of our mass spectrometry systems are therefore a top priority. A varied selection of our recent projects using ambient ionization, direct analysis in real time (DART), and solid phase microextraction (SPME) to analyze ancient, historic, and modern objects is presented, as well as some of our instrumental modifications to accommodate the unique needs of a museum.

November 2019 Meeting

Speaker: Xiaoyu Yang, National Institute of Standards and Technology

Topic: Searching a Comprehensive High Resolution Tandem Mass Spectral Library for Accurate Metabolite Identification

Date: Monday, November 18th, 2019

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instruments, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Meghan Burke (meghan.burke@nist.gov) by Friday, November 15th if you will be attending the dinner.

Abstract: Identifying metabolites from millions of electrospray-generated tandem mass spectra is highly challenging, especially in view of the complex chemistry occurring in the electrospray process. We are working to assist in the identification process through the development of a comprehensive reference tandem mass spectral library. We will describe our two part approach. First, we are rapidly building a fully annotated library of all major ions generated in the electrospray process for compounds of analytical interest. Second, we are extending search algorithms to identify compounds not present in the library, but which have similar fragmentation mechanisms. We will describe our latest developments in these areas, with a focus on human metabolite identification.

October 2019 Meeting

Speaker: Leslie Hicks, University of North Carolina at Chapel Hill

Topic: Investigating plant-derived antimicrobial peptides using PepSAVI-MS

Date: Monday, October 21st, 2019

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instruments, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Meghan Burke (meghan.burke@nist.gov) by Friday, October 18th if you will be attending the dinner.

Abstract: As current methods for antibiotic drug discovery are being outpaced by the rise of antimicrobial resistance, new methods and innovative technologies are crucial to replenish our dwindling arsenal of antimicrobial therapeutics. While natural products are a well-studied source of biologically active small molecules, peptidyl factors contributing to their medicinal properties remain largely unexplored. To this end, we have developed the PepSAVI-MS to identify bioactive peptide targets from complex biological samples. The developed platform is highly versatile as it is adaptable to any natural product source of peptides and can test against diverse physiological targets, including bacteria, fungi, and cancer cells for which there is a developed bioassay. As such, we demonstrated extension of this pipeline to fungal and bacterially-sourced AMPs and are beginning to probe the vast array of botanical natural product sources to prioritize highly active species for downstream analysis.

Burnaby Munson Memorial at U. Delaware

Celebration of Dr. Munson’s life set Oct. 4 in Mitchell Hall

(from the UDaily)

The life of Burnaby Munson, the C. Eugene Bennett Chair Emeritus of Chemistry and former director of the University of Delaware Honors Program, will be celebrated at a special event at 4 p.m., Friday, Oct. 4, in Mitchell Hall.

Dr. Munson died on June 23. He was 86.

All members of the UD community are invited to join in remembering Dr. Munson and sharing memories and moments from his legacy.

The event will be live streamed at https://sites.udel.edu/udlive.

Afterward, a reception will be held at the Honors Program, 186 South College Ave., Newark DE.

September 2019 Meeting

Speaker: Joseph Zaia, Boston University School of Medicine

Topic: Proteomics, glycomics, and glycoproteomics of matrisome molecules

Date: Monday, September 16th, 2019

Time: 6:15 pm Dinner, 7:15 pm Presentation

Location: Shimadzu Scientific Instruments, Inc. Training Center 7100 Riverwood Drive, Columbia, MD 21046 (Directions)

Dinner: Please RSVP to Meghan Burke (meghan.burke@nist.gov) by Friday, September 16th if you will be attending the dinner.

Abstract: The most straightforward applications of proteomics database searching involve intracellular proteins. While intracellular gene products number in the thousands, their well-defined post-translational modifications (PTMs) makes database searching practical. By contrast, cell surface and extracellular matrisome proteins pass through the secretory pathway where many become glycosylated, modulating their physicochemical properties, adhesive interactions, and diversifying their functions. While matrisome proteins number only a few hundred, their high degree of complex glycosylation multiplies the number of theoretical proteoforms by orders of magnitude. Given that extracellular networks that mediate cell-cell and cell-pathogen interactions in physiology depend on glycosylation, it is important to characterize the proteomes, glycomes and glycoproteomes of matrisome molecules that exist in a given biological context. In this presentation, I will summarize proteomics approaches for characterizing matrisome molecules, with an emphasis on applications to brain diseases.